Cloned (Comment) | Organism |
---|---|
gene MJ0026, expression in Escherichia coli strain BL21-CodonPlus(DE3)-RIL | Methanocaldococcus jannaschii |
Crystallization (Comment) | Organism |
---|---|
Trm4 free and in complex with sinefungin, hanging-drop vapor diffusion, 0.001 ml of protein solution containing 10.8 mg/ml protein with 0.001 ml of crystallization solution containing 100 mM Tris-HCl, pH 7.4, 200 mM MgCl2, 25% PEG 3350, and 6% v/v isopropanol for MJ0026 alone, and 20 100 mM Tris-HCl, pH 7.4, 200 mM MgCl2, 25% PEG 3350, 3 mM sinefungin, and 10 mM cytidine for Mj0026 with sinefungin, cryoprotectant 20% glycerol and 20% PEG 400, respectively, X-ray diffraction structure determination and analysis at 1.27 A and 2.3 A resolutions, respectively | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cytosine40 in tRNA precursor | Methanocaldococcus jannaschii | - |
S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA precursor | - |
? | |
S-adenosyl-L-methionine + cytosine48 in tRNA | Methanocaldococcus jannaschii | - |
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
gene MJ0026 | - |
Purification (Comment) | Organism |
---|---|
recombinant MJ0026 from Escherichia coli strain BL21-CodonPlus(DE3)-RIL by heat treatment, anion exchange and hydroxyapatite chromatography, and dialysis | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | highly conserved residues form a large, positively charged surface, which seems to be suitable for tRNA binding. Substrate specificity and methylation sites on tRNA, overview | Methanocaldococcus jannaschii | ? | - |
? | |
S-adenosyl-L-methionine + cytosine40 in tRNA precursor | - |
Methanocaldococcus jannaschii | S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA precursor | - |
? | |
S-adenosyl-L-methionine + cytosine48 in tRNA | - |
Methanocaldococcus jannaschii | S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MJ0026 | - |
Methanocaldococcus jannaschii |
RNA:m5C methyltransferase | - |
Methanocaldococcus jannaschii |
TRM4 | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
assay at | Methanocaldococcus jannaschii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the S-adenosyl-L-methionine analigue sinefungin is bound in a negatively charged pocket near helix alpha8, binding structure, detailed overview | Methanocaldococcus jannaschii | |
S-adenosyl-L-methionine | helix alpha8 can adopt two different conformations, thereby controlling the entry of S-adenosyl-L-methionine into the active site | Methanocaldococcus jannaschii |