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Literature summary for 2.1.1.202 extracted from

  • Jiang, H.Q.; Motorin, Y.; Jin, Y.X.; Grosjean, H.
    Pleiotropic effects of intron removal on base modification pattern of yeast tRNAPhe: an in vitro study (1997), Nucleic Acids Res., 25, 2694-2701.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.202

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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Saccharomyces cerevisiae Pp1001
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + cytosine40 in tRNA precursor the enzymatic formation of m5C at position 40 in the anticodon stem is strictly dependent on the presence of the intron. Enzymatic formation of m5C40 is independent of the whole architecture of the tRNA molecule and takes place on a minisubstrate composed of the anticodon stem nd loop of yeast tRNAPhe prolonged by its natural 19 nt intron Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA precursor
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 ?
S-adenosyl-L-methionine + cytosine40 in tRNA precursor the enzymatic formation of m5C at position 40 in the anticodon stem is strictly dependent on the presence of the intron. Enzymatic formation of m5C40 is independent of the whole architecture of the tRNA molecule and takes place on a minisubstrate composed of the anticodon stem nd loop of yeast tRNAPhe prolonged by its natural 19 nt intron Saccharomyces cerevisiae Pp1001 S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA precursor
-
 ?
S-adenosyl-L-methionine + cytosine49 in tRNA yeast tRNA(GAA) Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
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 ?
S-adenosyl-L-methionine + cytosine49 in tRNA yeast tRNA(GAA) Saccharomyces cerevisiae Pp1001 S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
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 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Saccharomyces cerevisiae