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Literature summary for 2.1.1.200 extracted from

  • Purta, E.; van Vliet, F.; Tkaczuk, K.L.; Dunin-Horkawicz, S.; Mori, H.; Droogmans, L.; Bujnicki, J.M.
    The yfhQ gene of Escherichia coli encodes a tRNA:Cm32/Um32 methyltransferase (2006), BMC Mol. Biol., 7, 23.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.200

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29168
-
 2 * 29168, calculated from sequence Escherichia coli
30000
-
 2 * 30000, SDS-PAGE in presence of 7.5% 2-mercaptoethanol Escherichia coli
58000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + cytidine32 in tRNA Escherichia coli presence of 2'-O-methylated cytidine at position 32 in tRNAfMet1(CAU), tRNAfMet2(CAU), tRNASer1(UGA), and tRNATrp1(CCA). In Escherichia coli YfhQ is the only methyltransferase responsible for the formation of Cm32 in tRNA S-adenosyl-L-homocysteine + 2'-O-methylcytidine32 in tRNA
-
 ?
S-adenosyl-L-methionine + uridine32 in tRNA Escherichia coli presence of 2'-O-methylated uridine in position 32 of the anticodon loop in tRNAGln1(UUG) and tRNAGln2(CUG) S-adenosyl-L-homocysteine + 2'-O-methyluridine32 in tRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AE01
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no methylation of cytosine 34 in tRNALeu(CAA) Escherichia coli ?
-
 ?
S-adenosyl-L-methionine + cytidine32 in tRNA presence of 2'-O-methylated cytidine at position 32 in tRNAfMet1(CAU), tRNAfMet2(CAU), tRNASer1(UGA), and tRNATrp1(CCA). In Escherichia coli YfhQ is the only methyltransferase responsible for the formation of Cm32 in tRNA Escherichia coli S-adenosyl-L-homocysteine + 2'-O-methylcytidine32 in tRNA
-
 ?
S-adenosyl-L-methionine + cytidine32 in tRNA 2'-O-methylation of cytidine34 in tRNAGln2(CUG). Total crude tRNA extracted from the wild-type strain MC1061 was not a substrate for the purified YfhQ enzyme, while tRNA from the yfhQ K.O. strain is an excellent substrate for this enzyme Escherichia coli S-adenosyl-L-homocysteine + 2'-O-methylcytidine32 in tRNA
-
 ?
S-adenosyl-L-methionine + uridine32 in tRNA presence of 2'-O-methylated uridine in position 32 of the anticodon loop in tRNAGln1(UUG) and tRNAGln2(CUG) Escherichia coli S-adenosyl-L-homocysteine + 2'-O-methyluridine32 in tRNA
-
 ?
S-adenosyl-L-methionine + uridine32 in tRNA 2'-O-methylation of uridine34 tRNASer1(UGA). Total crude tRNA extracted from the wild-type strain MC1061 was not a substrate for the purified YfhQ enzyme, while tRNA from the yfhQ K.O. strain is an excellent substrate for this enzyme Escherichia coli S-adenosyl-L-homocysteine + 2'-O-methyluridine32 in tRNA
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 29168, calculated from sequence Escherichia coli
dimer 2 * 30000, SDS-PAGE in presence of 7.5% 2-mercaptoethanol Escherichia coli

Synonyms

Synonyms Comment Organism
TrMet(Xm32)
-
 Escherichia coli
TrmJ
-
 Escherichia coli
tRNA:Cm32/Um32 methyltransferase
-
 Escherichia coli
YfhQ
-
 Escherichia coli