Literature summary for 2.1.1.20 extracted from

Luka, Z.; Pakhomova, S.; Luka, Y.; Newcomer, M.E.; Wagner, C.
Destabilization of human glycine N-methyltransferase by H176N mutation (2007), Protein Sci., 16, 1957-1964.

Search for more literature for 2.1.1.20

Cloned(Commentary)

Cloned (Comment)	Organism
wild type and H176N mutant enzyme	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
wild type and H176N mutant enzyme as cocrystals with a citrate molecule bound in the active site	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
H176N	naturally occurring mutation, 25% less activity and highly reduced stability compared with the native enzyme, completely inactive at 2 M urea compared with 60% remaining activity of the wild type enzyme	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	major folate binding protein	?	-	?
S-adenosyl-L-methionine + glycine	Homo sapiens	key enzyme for the regulation of the ratio of S-adenosylmethionine to S-adenosylhomocysteine	S-adenosyl-L-homocysteine + N-methylglycine	N-methylglycine = sarcosine	?

Organic Solvent Stability

Organic Solvent	Comment	Organism
urea	H176N mutant completely inactive at 2 M urea compared with 60% remaining activity of the wild type enzyme	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q14749	normal enzyme and naturally occurring H176N mutation which is found in humans with hypermethioninaemia	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzymes	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	major folate binding protein	Homo sapiens	?	-	?
S-adenosyl-L-methionine + glycine	-	Homo sapiens	S-adenosyl-L-homocysteine + N-methylglycine	N-methylglycine = sarcosine	?
S-adenosyl-L-methionine + glycine	key enzyme for the regulation of the ratio of S-adenosylmethionine to S-adenosylhomocysteine	Homo sapiens	S-adenosyl-L-homocysteine + N-methylglycine	N-methylglycine = sarcosine	?

Subunits

Subunits	Comment	Organism
tetramer	crystal structure analysis, in the presence of moderate (2-4 M) urea concentrations the tetrameric enzyme dissociates into compact monomers, higher concentrations of urea (7-8 M) promote complete denaturation of enzyme	Homo sapiens

Synonyms

Synonyms	Comment	Organism
GNMT	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay temperature	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay pH	Homo sapiens

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Release 2023.1 (January 2023)