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Literature summary for 2.1.1.2 extracted from

  • Takata, Y.; Konishi, K.; Gomi, T.; Fujioka, M.
    Rat guanidinoacetate methyltransferase. Effect of site-directed alteration of an aspartic acid residue that is conserved across most mammalian S-adenosylmethionine-dependent methyltransferases (1994), J. Biol. Chem., 269, 5537-5542.
    View publication on PubMed
Search for more literature for 2.1.1.2

Protein Variants

Protein Variants Comment Organism
D129A active enzyme Rattus norvegicus
D129N active enzyme Rattus norvegicus
D134A inactive Rattus norvegicus
D134E lower activity than wild type enzyme Rattus norvegicus
D134N much lower activity than wild type enzyme Rattus norvegicus
T135A lower activity than wild type enzyme Rattus norvegicus
Y133F lower activity than wild type enzyme Rattus norvegicus
Y136F higher turnover than wild type enzyme Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information all mutant enzymes listed with Km Rattus norvegicus
0.007
-
 S-adenosyl-L-methionine wild type enzyme Rattus norvegicus
0.011
-
 Guanidinoacetate wild type enzyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + guanidinoacetate Rattus norvegicus
-
 S-adenosyl-L-homocysteine + creatine
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P10868
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + guanidinoacetate
-
 Rattus norvegicus S-adenosyl-L-homocysteine + creatine
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information all mutant enzymes listed with turnover Rattus norvegicus
0.081
-
 Guanidinoacetate wild type enzyme Rattus norvegicus