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Literature summary for 2.1.1.2 extracted from
- Guanidoacetate methyltransferase from rat liver: purification, properties, and evidence for the involvement of sulfhydryl groups for activity (1983), Arch. Biochem. Biophys., 226, 265-275.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Rattus norvegicus |  |
| cysteine | - |
Rattus norvegicus | |
| dithiothreitol | - |
Rattus norvegicus | |
| glutathione | required for maximum activity, can be replaced by other reducing agents | Rattus norvegicus | |
General Stability
| General Stability | Organism |
|---|---|
| inactivated during purification, can be reactivated by addition of a thiol | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| glutathione | oxidized form, S-adenosylmethionine protects against inactivation | Rattus norvegicus | |
| N-ethylmaleimide | irreversible | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
S-adenosyl-L-methionine | - |
Rattus norvegicus | |
| 0.098 | - |
Guanidinoacetate | - |
Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
gel filtration | Rattus norvegicus |
| 26000 | - |
SDS-PAGE, in presence and absence of SDS | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + guanidinoacetate | Rattus norvegicus | - |
S-adenosyl-L-homocysteine + creatine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| inactivated during purification, can be reactivated by addition of a thiol | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.16 | - |
purified enzyme | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 10 mM phosphate buffer, pH 7.2, 1 mM EDTA, 0.1 M NaCl, concentrated by ultrafiltration | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + guanidinoacetate | - |
Rattus norvegicus | S-adenosyl-L-homocysteine + creatine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | SDS-PAGE, gel filtration | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
remains active for 24 h | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Rattus norvegicus |
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