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Literature summary for 2.1.1.197 extracted from
- The BioC O-methyltransferase catalyzes methyl esterification of malonyl-acyl carrier protein, an essential step in biotin synthesis (2012), J. Biol. Chem., 287, 37010-37020.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Bacillus cereus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibitory: 5'-deoxyadenosine, methylthioadenosine, adenosine, L-homocysteine | Bacillus cereus | |
| S-adenosyl-L-homocysteine | product inhibition | Bacillus cereus |  |
| sinefungin | 0.1 micromol, 60% reduction of activity | Bacillus cereus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00108 | - |
malonyl-[acyl-carrier protein] | pH 7, 37°C | Bacillus cereus | |
| 0.0019 | - |
S-adenosyl-L-methionine | pH 7, 37°C, cosubstrate malonyl-[acyl-carrier protein] | Bacillus cereus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | addition of metal ions neither enhances nor reduces the activity | Bacillus cereus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + malonyl-[acyl-carrier protein] | Bacillus cereus | - |
S-adenosyl-L-homocysteine + malonyl-[acyl-carrier protein] methyl ester | - |
? | |
| S-adenosyl-L-methionine + malonyl-[acyl-carrier protein] | Bacillus cereus ATCC 10987 | - |
S-adenosyl-L-homocysteine + malonyl-[acyl-carrier protein] methyl ester | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | Q731I1 | - |
- |
| Bacillus cereus ATCC 10987 | Q731I1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus cereus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | holo-[acyl-carrier protein], acetyl-[acyl-carrier protein], succinyl-[acyl-carrier protein], and glutaryl-[acyl-carrier protein] are no substrates | Bacillus cereus | ? | - |
? | |
| additional information | holo-[acyl-carrier protein], acetyl-[acyl-carrier protein], succinyl-[acyl-carrier protein], and glutaryl-[acyl-carrier protein] are no substrates | Bacillus cereus ATCC 10987 | ? | - |
? | |
| S-adenosyl-L-methionine + malonyl-CoA | - |
Bacillus cereus | S-adenosyl-L-homocysteine + malonyl-CoA methyl ester | - |
? | |
| S-adenosyl-L-methionine + malonyl-CoA | - |
Bacillus cereus ATCC 10987 | S-adenosyl-L-homocysteine + malonyl-CoA methyl ester | - |
? | |
| S-adenosyl-L-methionine + malonyl-[acyl-carrier protein] | - |
Bacillus cereus | S-adenosyl-L-homocysteine + malonyl-[acyl-carrier protein] methyl ester | - |
? | |
| S-adenosyl-L-methionine + malonyl-[acyl-carrier protein] | acyl-carrier protein from Escherichia coli | Bacillus cereus | S-adenosyl-L-homocysteine + malonyl-[acyl-carrier protein] methyl ester | - |
? | |
| S-adenosyl-L-methionine + malonyl-[acyl-carrier protein] | - |
Bacillus cereus ATCC 10987 | S-adenosyl-L-homocysteine + malonyl-[acyl-carrier protein] methyl ester | - |
? | |
| S-adenosyl-L-methionine + malonyl-[acyl-carrier protein] | acyl-carrier protein from Escherichia coli | Bacillus cereus ATCC 10987 | S-adenosyl-L-homocysteine + malonyl-[acyl-carrier protein] methyl ester | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 173 | - |
S-adenosyl-L-methionine | pH 7, 37°C, cosubstrate malonyl-[acyl-carrier protein] | Bacillus cereus | |
| 225 | - |
malonyl-[acyl-carrier protein] | pH 7, 37°C | Bacillus cereus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Bacillus cereus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7.5 | less than 50% of maximal activity above and below | Bacillus cereus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 20830 | - |
S-adenosyl-L-methionine | pH 7, 37°C, cosubstrate malonyl-[acyl-carrier protein] | Bacillus cereus | |
| 91050 | - |
malonyl-[acyl-carrier protein] | pH 7, 37°C | Bacillus cereus | |
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