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Literature summary for 2.1.1.192 extracted from

  • Challand, M.R.; Salvadori, E.; Driesener, R.C.; Kay, C.W.; Roach, P.L.; Spencer, J.
    Cysteine methylation controls radical generation in the Cfr radical AdoMet rRNA methyltransferase (2013), PLoS ONE, 8, e67979.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.192

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta2(DE3)pLysS cells Mammaliicoccus sciuri

Protein Variants

Protein Variants Comment Organism
C338A the mutant binds S-adenosyl-L-methionine with wild type affinity, while oxidation of the [4Fe-4S] cluster is not observed Mammaliicoccus sciuri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41085
-
 x * 41085, electrospray mass spectrometry Mammaliicoccus sciuri
41088
-
 x * 41088, fully methylated protein, calculated from amino acid sequence Mammaliicoccus sciuri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + adenine2503 in 23S rRNA Mammaliicoccus sciuri
-
 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Mammaliicoccus sciuri Q9FBG4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
 Mammaliicoccus sciuri S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA
-
 ?

Subunits

Subunits Comment Organism
? x * 41085, electrospray mass spectrometry Mammaliicoccus sciuri
? x * 41088, fully methylated protein, calculated from amino acid sequence Mammaliicoccus sciuri

Synonyms

Synonyms Comment Organism
Cfr
-
 Mammaliicoccus sciuri
radical AdoMet rRNA methyltransferase
-
 Mammaliicoccus sciuri

Cofactor

Cofactor Comment Organism Structure
[4Fe-4S]-center the enzyme contains a [4Fe-4S] cluster Mammaliicoccus sciuri