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Literature summary for 2.1.1.191 extracted from
- YccW is the m5C methyltransferase specific for 23S rRNA nucleotide 1962 (2008), J. Mol. Biol., 383, 641-651.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| full-length YccW protein with a C-terminal histidine tag is expressed in Escherichia coli | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46500 | - |
x * 46500, SDS-PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P75876 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + cytosine1962 in 23S rRNA | the enzyme can methylate naked 23S rRNA, but not assembled 50S ribosomal subunits or 70S ribosomes | Escherichia coli | S-adenosyl-L-homocysteine + 5-methylcytosine1962 in 23S rRNA | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 46500, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| YccW | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Escherichia coli |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | YccW directed methylation at nucleotide C1962 is conserved in bacteria | Escherichia coli |
| malfunction | inactivation of the yccW gene leads to loss of methylation at nucleotide 1962 in Escherichia coli 23S rRNA. This methylation is restored by complementing the knockout strain with a plasmid-encoded copy of the yccW gene. Loss of this methylation in Escherichia coli results in a slight growth defect compared to wild-type cells during competitive growth experiments in rich or minimal medium | Escherichia coli |
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Release 2023.1 (January 2023)
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