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Literature summary for 2.1.1.190 extracted from

  • Desmolaize, B.; Fabret, C.; Bregeon, D.; Rose, S.; Grosjean, H.; Douthwaite, S.
    A single methyltransferase YefA (RlmCD) catalyses both m5U747 and m5U1939 modifications in Bacillus subtilis 23S rRNA (2011), Nucleic Acids Res., 39, 9368-9375.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene yefA, phylogenetic analysis, functional complementation of Bacillus subtilis knockout strain BG12826 yefA::pMutin2 and DELTAyefA::Cm deletion mutant, and of Escherichia coli enzyme-deficient BW 25113 DELTAtrmA/DELTArlmC/DELTArlmD strain Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bacillus subtilis methylation of U747 and U1939 in Bacillus subtilis rRNA is catalysed by a single enzyme, YefA, MALDI mass spectrometry product identification. YefA is not involved in tRNA methylation, or in 23S rRNA m554 methylation ?
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S-adenosyl-L-methionine + uracil1939 in 23S rRNA Bacillus subtilis
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S-adenosyl-L-homocysteine + 5-methyluracil1939 in 23S rRNA
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?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information methylation of U747 and U1939 in Bacillus subtilis rRNA is catalysed by a single enzyme, YefA, MALDI mass spectrometry product identification. YefA is not involved in tRNA methylation, or in 23S rRNA m554 methylation Bacillus subtilis ?
-
?
S-adenosyl-L-methionine + uracil1939 in 23S rRNA
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Bacillus subtilis S-adenosyl-L-homocysteine + 5-methyluracil1939 in 23S rRNA
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?

Synonyms

Synonyms Comment Organism
RlmCD
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Bacillus subtilis
YefA
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Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
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Bacillus subtilis

General Information

General Information Comment Organism
evolution YefA is a COG2265 member. During evolution, COG2265 enzymes have undergone a series of changes in target specificity and YefA is closer to an archetypical m5U methyltransferase. A functional shift has occurred during the evolution of the Bacillus subtilis YefA and YfjO methyltransferases.To reflect its dual specificity, YefA is renamed RlmCD Bacillus subtilis