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Literature summary for 2.1.1.190 extracted from

  • Madsen, C.T.; Mengel-Jorgensen, J.; Kirpekar, F.; Douthwaite, S.
    Identifying the methyltransferases for m(5)U747 and m(5)U1939 in 23S rRNA using MALDI mass spectrometry (2003), Nucleic Acids Res., 31, 4738-4746.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of a intein-fusion protein with an C-terminal His-tag Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P55135
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-

Purification (Commentary)

Purification (Comment) Organism
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Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + uracil1939 in 23S rRNA a purified recombinant version of YgcA retains its activity and specificity, and methylates U1939 in an RNA transcript in vitro Escherichia coli S-adenosyl-L-homocysteine + 5-methyluracil1939 in 23S rRNA
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Synonyms

Synonyms Comment Organism
RNA uridine methyltransferase A
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Escherichia coli
RumA
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Escherichia coli
YgcA
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Escherichia coli