Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.190 extracted from

  • Agarwalla, S.; Kealey, J.T.; Santi, D.V.; Stroud, R.M.
    Characterization of the 23 S ribosomal RNA m5U1939 methyltransferase from Escherichia coli (2002), J. Biol. Chem., 277, 8835-8840.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
uracil1939 in 23S rRNA pH 7.5, 37°C, uracil1939 in 40-nucleotide 23 S rRNA fragment (nucleotide 1930–1969) Escherichia coli
0.026
-
S-adenosyl-L-methionine pH 7.5, 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Iron the enzyme contains two equivalents of iron/monomer and has a sequence motif similar to a motif found in iron-sulfur proteins Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P55135
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + uracil1939 in 23S rRNA the enzyme specifically methylates uracil1939 at C5 in 23S rRNA. A 40-nucleotide 23 S rRNA fragment (nucleotide 1930–1969) also serves as an efficient substrate for the enzyme Escherichia coli S-adenosyl-L-homocysteine + 5-methyluracil1939 in 23S rRNA identification of C5-uracil1939 as reaction product ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.06
-
S-adenosyl-L-methionine pH 7.5, 37°C Escherichia coli
0.06
-
uracil1939 in 23S rRNA pH 7.5, 37°C, uracil1939 in 40-nucleotide 23 S rRNA fragment (nucleotide 1930–1969) Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli