Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
uracil1939 in 23S rRNA | pH 7.5, 37°C, uracil1939 in 40-nucleotide 23 S rRNA fragment (nucleotide 19301969) | Escherichia coli | |
0.026 | - |
S-adenosyl-L-methionine | pH 7.5, 37°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the enzyme contains two equivalents of iron/monomer and has a sequence motif similar to a motif found in iron-sulfur proteins | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P55135 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + uracil1939 in 23S rRNA | the enzyme specifically methylates uracil1939 at C5 in 23S rRNA. A 40-nucleotide 23 S rRNA fragment (nucleotide 19301969) also serves as an efficient substrate for the enzyme | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluracil1939 in 23S rRNA | identification of C5-uracil1939 as reaction product | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
S-adenosyl-L-methionine | pH 7.5, 37°C | Escherichia coli | |
0.06 | - |
uracil1939 in 23S rRNA | pH 7.5, 37°C, uracil1939 in 40-nucleotide 23 S rRNA fragment (nucleotide 19301969) | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |