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Literature summary for 2.1.1.184 extracted from

  • Schluckebier, G.; Zhong, P.; Stewart, K.D.
    Kavanaugh, T.J.; Abad-Zapatero, C.: The 2.2 A structure of the rRNA methyltransferase ErmC' and its complexes with cofactor and cofactor analogs: implications for the reaction mechanism (1999), J. Mol. Biol., 289, 277-291.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallized by the hanging drop vapor diffusion method. Structure of the apo-enzyme at 2.2 A resolution. The crystal structures of ErmC' and of its complexes with the cofactor S-adenosyl-L-methionine, the reaction product S-adenosyl-L-homocysteine and the methyltransferase inhibitor sinefungin, respectively, show that the enzyme undergoes small conformational changes upon ligand binding Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
S-adenosyl-L-homocysteine
-
Bacillus subtilis
sinefungin competitive Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
S-adenosyl-L-methionine pH 7.5, 21°C Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 S-adenosyl-L-methionine + adenine2085 in 23S rRNA Bacillus subtilis the rRNA methyltransferase ErmC0 transfers methyl groups from S-adenosyl-L-methionine to atom N6 of an adenine base within the peptidyltransferase loop of 23 S rRNA, thus conferring antibiotic resistance against a number of macrolide antibiotics 2 S-adenosyl-L-homocysteine + N6-dimethyladenine2085 in 23S rRNA
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P13956
-
-

Reaction

Reaction Comment Organism Reaction ID
2 S-adenosyl-L-methionine + adenine2085 in 23S rRNA = 2 S-adenosyl-L-homocysteine + N6-dimethyladenine2085 in 23S rRNA a model for the transition-state based on the atomic details of the active site is consistent with a one-step methyl-transfer Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + adenine2085 in 23S rRNA
-
Bacillus subtilis 2 S-adenosyl-L-homocysteine + N6-dimethyladenine2085 in 23S rRNA
-
?
2 S-adenosyl-L-methionine + adenine2085 in 23S rRNA the rRNA methyltransferase ErmC0 transfers methyl groups from S-adenosyl-L-methionine to atom N6 of an adenine base within the peptidyltransferase loop of 23 S rRNA, thus conferring antibiotic resistance against a number of macrolide antibiotics Bacillus subtilis 2 S-adenosyl-L-homocysteine + N6-dimethyladenine2085 in 23S rRNA
-
?

Synonyms

Synonyms Comment Organism
rRNA methyltransferase ErmC'
-
Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Bacillus subtilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.018
-
sinefungin pH 7.5, 21°C Bacillus subtilis
0.033
-
S-adenosyl-L-homocysteine pH 7.5, 21°C Bacillus subtilis

General Information

General Information Comment Organism
physiological function transfers methyl groups from S-adenosyl-l-methionine to atom N6 of an adenine base within the peptidyltransferase loop of 23S rRNA, thus conferring antibiotic resistance against a number of macrolide antibiotics Bacillus subtilis