Crystallization (Comment) | Organism |
---|---|
crystallized by the hanging drop vapor diffusion method. Structure of the apo-enzyme at 2.2 A resolution. The crystal structures of ErmC' and of its complexes with the cofactor S-adenosyl-L-methionine, the reaction product S-adenosyl-L-homocysteine and the methyltransferase inhibitor sinefungin, respectively, show that the enzyme undergoes small conformational changes upon ligand binding | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-homocysteine | - |
Bacillus subtilis | |
sinefungin | competitive | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
S-adenosyl-L-methionine | pH 7.5, 21°C | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + adenine2085 in 23S rRNA | Bacillus subtilis | the rRNA methyltransferase ErmC0 transfers methyl groups from S-adenosyl-L-methionine to atom N6 of an adenine base within the peptidyltransferase loop of 23 S rRNA, thus conferring antibiotic resistance against a number of macrolide antibiotics | 2 S-adenosyl-L-homocysteine + N6-dimethyladenine2085 in 23S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P13956 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 S-adenosyl-L-methionine + adenine2085 in 23S rRNA = 2 S-adenosyl-L-homocysteine + N6-dimethyladenine2085 in 23S rRNA | a model for the transition-state based on the atomic details of the active site is consistent with a one-step methyl-transfer | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + adenine2085 in 23S rRNA | - |
Bacillus subtilis | 2 S-adenosyl-L-homocysteine + N6-dimethyladenine2085 in 23S rRNA | - |
? | |
2 S-adenosyl-L-methionine + adenine2085 in 23S rRNA | the rRNA methyltransferase ErmC0 transfers methyl groups from S-adenosyl-L-methionine to atom N6 of an adenine base within the peptidyltransferase loop of 23 S rRNA, thus conferring antibiotic resistance against a number of macrolide antibiotics | Bacillus subtilis | 2 S-adenosyl-L-homocysteine + N6-dimethyladenine2085 in 23S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
rRNA methyltransferase ErmC' | - |
Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Bacillus subtilis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
sinefungin | pH 7.5, 21°C | Bacillus subtilis | |
0.033 | - |
S-adenosyl-L-homocysteine | pH 7.5, 21°C | Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
physiological function | transfers methyl groups from S-adenosyl-l-methionine to atom N6 of an adenine base within the peptidyltransferase loop of 23S rRNA, thus conferring antibiotic resistance against a number of macrolide antibiotics | Bacillus subtilis |