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Literature summary for 2.1.1.182 extracted from

  • Poldermans, B.; Roza, L.; van Knippenberg, P.H.
    Studies on the function of two adjacent N6,N6-dimethyladenosines near the 3' end of 16 S ribosomal RNA of Escherichia coli. III. Purification and properties of the methylating enzyme and methylase-30 S interactions (1979), J. Biol. Chem., 254, 9094-9100.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
additional information a mixture of 30 S ribosomal proteins inhibits methylation of mutant 30 S ribosomes. This inhibition can be ascribed to ribosomal protein S21. Initiation factor 3 partially inhibits methylation of mutant 30 S ribosomes Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 2-3 mM, required Escherichia coli
additional information K+ and NH4+ in the range of 10 to 150 mM have little effect on methylation Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4 S-adenosyl-L-methionine + adenine1518/adenine1519 in 16S rRNA
-
 Escherichia coli 4 S-adenosyl-L-homocysteine + N6-dimethyladenine1518/N6-dimethyladenine1519 in 16S rRNA
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 30000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6.8 7.8 change of pH from 7.8 to 6.8 reduces methylation by 50% Escherichia coli