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Literature summary for 2.1.1.179 extracted from

  • Gutierrez, B.; Escudero, J.A.; San Millan, A.; Hidalgo, L.; Carrilero, L.; Ovejero, C.M.; Santos-Lopez, A.; Thomas-Lopez, D.; Gonzalez-Zorn, B.
    Fitness cost and interference of Arm/Rmt aminoglycoside resistance with the RsmF housekeeping methyltransferases (2012), Antimicrob. Agents Chemother., 56, 2335-2341.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.179

Cloned(Commentary)

Cloned (Comment) Organism
gene rmtC, expression of wild-type enzyme in Escherichi coli Top10 cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information construction of a series of in-frame knockout and knock-in mutants of Escherichia coli, corresponding to the genotypes rsmF+, DELTArsmF, rsmF+ rmtC+, and DELTArsmF rmtC+, mutant strain growth kinetics, overview Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli RmtC in the presence of aminoglycosides impedes methylation at the N5 position of nucleotide C1407 when the N7 position of G1405 is methylated, MALDI mass spectrometry product analysis, mechanism, overview ?
-
 ?
additional information Escherichia coli BW25113 RmtC in the presence of aminoglycosides impedes methylation at the N5 position of nucleotide C1407 when the N7 position of G1405 is methylated, MALDI mass spectrometry product analysis, mechanism, overview ?
-
 ?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA Escherichia coli
-
 S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
 ?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA Escherichia coli BW25113
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 S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 i.e. strain BB1074, gene rmtC
-
Escherichia coli BW25113
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 i.e. strain BB1074, gene rmtC
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information RmtC in the presence of aminoglycosides impedes methylation at the N5 position of nucleotide C1407 when the N7 position of G1405 is methylated, MALDI mass spectrometry product analysis, mechanism, overview Escherichia coli ?
-
 ?
additional information RmtC in the presence of aminoglycosides impedes methylation at the N5 position of nucleotide C1407 when the N7 position of G1405 is methylated, MALDI mass spectrometry product analysis, mechanism, overview Escherichia coli BW25113 ?
-
 ?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
 Escherichia coli S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
 ?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
 Escherichia coli BW25113 S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
 ?

Synonyms

Synonyms Comment Organism
methyltransferase RmtC
-
 Escherichia coli
RmtC
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Escherichia coli

General Information

General Information Comment Organism
additional information acquisition of rmtC does not entail a fitness cost for the bacterium Escherichia coli
physiological function the enzyme confers high-level resistance to 4,6-disubstituted aminoglycosides through methylation of the G1405 residue in the 16S rRNA. RmtC impedes methylation by the housekeeping methyltransferase RsmF, EC 2.1.1.178, at position C1407 Escherichia coli