Cloned (Comment) | Organism |
---|---|
cloning of the sgm gene into pET-25b (+) vector with the addition of N-terminal non-cleavable His6 tag, as well as alanine mutagenesis of residues D156, D182 and R108. The constructs are co-transformed along with pGroESL into the strain BL21 (DE3) of Escherichia coli for protein expression | Micromonospora zionensis |
Crystallization (Comment) | Organism |
---|---|
purified Sgm is complexed with 5 mM of the cofactors S-adenosylmethionine/S-adenosylhomocysteine. Crystallization trials are carried out at room temperature by hanging-drop vapor-diffusion method, structure of Sgm in complex with cofactors S-adenosylmethionine and S-adenosylhomocysteine is determined at 2.0 A and 2.1 A resolution, respectively | Micromonospora zionensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine1405 in 16S rRNA | Micromonospora zionensis | the enzyme confers resistance to aminoglycosides like gentamicin and sisomicin by specifically methylating G1405 in bacterial 16S rRNA | S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Micromonospora zionensis | Q7M0R2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Micromonospora zionensis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + guanine1405 in 16S rRNA = S-adenosyl-L-homocysteine + N7-methylguanine1405 in 16S rRNA | the mechanism of methylation of G1405 by Sgm is proposed and compared with other m7G methyltransferases | Micromonospora zionensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine1405 in 16S rRNA | - |
Micromonospora zionensis | S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine1405 in 16S rRNA | the enzyme confers resistance to aminoglycosides like gentamicin and sisomicin by specifically methylating G1405 in bacterial 16S rRNA | Micromonospora zionensis | S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
sgm | - |
Micromonospora zionensis |
sisomicin-gentamicin methyltransferase | - |
Micromonospora zionensis |