Cloned (Comment) | Organism |
---|---|
the Escherichia coli YebU gene is cloned into the PT73.3HisGW vector.25 The resulting construct encodes a polypeptide with the YebU gene and both N- and C-terminal hexa-histidine tails | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
YebU crystals are grown at room temperature by the hanging-drop, vapour-diffusion method, Crystal structure solved at 2.9 A resolution. The enzyme has an N-terminal catalytic domain that binds S-adenosylmethionine. The C-terminal domain has structural similarity to PUA domains of pseudouridine synthases and archaeosine-specific transglycosylases. The YebU structure is used to propose the most likely mechanism for its substrate specificity and recognition | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P76273 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + cytosine1407 in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA | the YebU structure is used to propose the most likely mechanism for its substrate specificity and recognition | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cytosine1407 in 16S rRNA | - |
Escherichia coli | S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNA m5C methyltransferase YebU | - |
Escherichia coli |
RsmF | - |
Escherichia coli |
YebU | - |
Escherichia coli |