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Literature summary for 2.1.1.177 extracted from
- Identification of pseudouridine methyltransferase in Escherichia coli (2008), RNA, 14, 2223-2233.
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + pseudouridine1915 in 23S rRNA | Escherichia coli | YbeA is acting at the final stage during ribosome assembly, probably during translation initiation | S-adenosyl-L-homocysteine + N3-methylpseudouridine1915 in 23S rRNA | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0A8I8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminal His6-tagged YbeA | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + pseudouridine1915 in 23S rRNA | YbeA is acting at the final stage during ribosome assembly, probably during translation initiation | Escherichia coli | S-adenosyl-L-homocysteine + N3-methylpseudouridine1915 in 23S rRNA | - |
? | |
| S-adenosyl-L-methionine + pseudouridine1915 in 23S rRNA | pseudouridine is the preferred substrate, inability of YbeA to methylate uridine at position 1915. The enzyme requires the intact ribosome and the presence of pseudouridine at position 1915 in 23S rRNA for activity | Escherichia coli | S-adenosyl-L-homocysteine + N3-methylpseudouridine1915 in 23S rRNA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| m3Psi methyltransferase | - |
Escherichia coli |
| pseudouridine methyltransferase | - |
Escherichia coli |
| Psi1915-specific methyltransferase | - |
Escherichia coli |
| RlmH | - |
Escherichia coli |
| YbeA | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Escherichia coli |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | the Escherichia coli ybeA gene deletion strain lacks the N3-methylation at position 1915 of 23S rRNA | Escherichia coli |
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Release 2023.1 (January 2023)
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