Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a ycbY knock-out strain, completely inactive in methylation, from Escherichia coli strain BW25113 and complementation with active ycbY | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | the bifunctional methyltransferase YcbY, i.e. RlmKL, adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA, recognition of dual rRNA targets by YcbY | ? | - |
? | |
S-adenosyl-L-methionine + guanine2069 in 23S rRNA | Escherichia coli | - |
S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine2445 in 23S rRNA | Escherichia coli | - |
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine2445 in 23S rRNA | Streptococcus mutans | Smu472 adds the m2G2445 modification | S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P75864 | gene ycbY | - |
Streptococcus mutans | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the bifunctional methyltransferase YcbY, i.e. RlmKL, adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA, recognition of dual rRNA targets by YcbY | Escherichia coli | ? | - |
? | |
additional information | Smu776 does not target G2069, and this nucleotide remains unmodified in Gram-positive rRNAs | Streptococcus mutans | ? | - |
? | |
S-adenosyl-L-methionine + guanine2069 in 23S rRNA | - |
Escherichia coli | S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine2069 in 23S rRNA | m7G2069 modification by YcbY is stoichiometric | Escherichia coli | S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine2445 in 23S rRNA | - |
Streptococcus mutans | S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine2445 in 23S rRNA | - |
Escherichia coli | S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine2445 in 23S rRNA | Smu472 adds the m2G2445 modification | Streptococcus mutans | S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine2445 in 23S rRNA | m2G2445 modification by YcbY is stoichiometric | Escherichia coli | S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure comparisosns of Streptococcus mutans proteins Smu472 and Smu776 with Escherichia coli RlmKL protein, the active site and their folding patterns respectively resemble each other, overview | Streptococcus mutans |
More | structure comparisosns with Streptococcus mutans proteins Smu472 and Smu776, the active site and their folding patterns respectively resemble each other, overview | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
m2G2445 methyltransferase | - |
Streptococcus mutans |
m2G2445 methyltransferase | - |
Escherichia coli |
ribosomal large subunit methyltransferase | - |
Escherichia coli |
RlmKL | - |
Escherichia coli |
Smu472 | - |
Streptococcus mutans |
ycbY | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Streptococcus mutans | |
S-adenosyl-L-methionine | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the COG1092 family, evolutionary implications of the apparent emergence of Escherichia coli YcbY from the fusion of Streptococcus mutans Smu472 and Smu776 orthologues are considered | Streptococcus mutans |
evolution | the enzyme is a member of the COG1092 family, evolutionary implications of the apparent emergence of Escherichia coli YcbY from the fusion of Streptococcus mutans Smu472 and Smu776 orthologues are considered | Escherichia coli |
physiological function | the N-terminal region of YcbY adds the m2G2445 modification, while the C-terminal region of YcbY is responsible for the m7G2069 methylation on the opposite side of the same helix, H74. YcbY enzyme is an example of a methyltransferase catalyzing two mechanistically different types of RNA modification | Escherichia coli |