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Literature summary for 2.1.1.173 extracted from

  • Kimura, S.; Ikeuchi, Y.; Kitahara, K.; Sakaguchi, Y.; Suzuki, T.; Suzuki, T.
    Base methylations in the double-stranded RNA by a fused methyltransferase bearing unwinding activity (2012), Nucleic Acids Res., 40, 4071-4085.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.173

Cloned(Commentary)

Cloned (Comment) Organism
gene rlmKL, DNA and amino acid sequence determination and analysis, gene rlmL/ycbY has been renamed rlmKL Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information generation of several base-flipping mutant enzymes lacking m2G2445 formation activity, overview Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + guanine2069 in 23S rRNA Escherichia coli
-
 S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA Escherichia coli
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 S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 gene rlmL/ycbY has been renamed rlmKL
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information helix 80 and the 12 nt ss region are critical sites necessary for m2G2445 and m7G2069 formation. Transcript 7, which lacks helix 80 and the 12 nt single-strand region, is not methylated at either position Escherichia coli ?
-
 ?
S-adenosyl-L-methionine + guanine2069 in 23S rRNA
-
 Escherichia coli S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
-
 Escherichia coli S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA duplex formation of H74 is not required for the m2G2445 formation. The 29-mer single-stranded transcript 6, which consists of residues C2422 to A2450, can form m2G2445 efficiently Escherichia coli S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
-
 ?

Subunits

Subunits Comment Organism
More RlmKL is a fused methyltransferase consisting of an N-terminal RlmL domain and a C-terminal RlmK domain Escherichia coli

Synonyms

Synonyms Comment Organism
m2G2445 methyltransferase
-
 Escherichia coli
RlmKL
-
 Escherichia coli
RlmL
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Escherichia coli

General Information

General Information Comment Organism
malfunction deletion of rlmL/ycbY results in a slight growth reduction phenotype Escherichia coli
additional information RlmKL is involved in the efficient assembly of 50S subunit in a mutant strain lacking an RNA helicase deaD Escherichia coli
physiological function cooperative methylation of helix 74 by RlmKL plays a key role in the efficient assembly of the 50S subunit, RlmKL enzyme is an example of a methyltransferase catalyzing two mechanistically different types of RNA modification. RlmKL has an unwinding activity of Helix 74, facilitating cooperative methylations of m7G2069 and m2G2445 during biogenesis of 50S subunit. Methyltransferase RlmL, encoded by rlmL/ycbY, catalyzes S-adenosyl-L-methionine-dependent m2G2445 formation Escherichia coli