Literature summary for 2.1.1.172 extracted from

Sunita, S.; Purta, E.; Durawa, M.; Tkaczuk, K.L.; Swaathi, J.; Bujnicki, J.M.; Sivaraman, J.
Functional specialization of domains tandemly duplicated within 16S rRNA methyltransferase RsmC (2007), Nucleic Acids Res., 35, 4264-4274.

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Cloned(Commentary)

Cloned (Comment)	Organism
the rsmC gene, cloned into pCA24N vector with a noncleavable N-terminal His6 tag	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, crystal structure of RsmC refined to 2.1 A resolution, reveals two homologous domains tandemly duplicated within a single polypeptide. Characterization of the function of the individual domains and identification of key residues involved in binding of rRNA and S-adenosyl-L-methionine, and in catalysis. It is discovered that one of the domains is important for the folding of the other. RsmC can be regarded as a model system for functional streamlining of domains accompanied by the development of dependencies concerning folding and stability	Escherichia coli

Crystallization (Comment)

Organism

hanging drop vapor diffusion method, crystal structure of RsmC refined to 2.1 A resolution, reveals two homologous domains tandemly duplicated within a single polypeptide. Characterization of the function of the individual domains and identification of key residues involved in binding of rRNA and S-adenosyl-L-methionine, and in catalysis. It is discovered that one of the domains is important for the folding of the other. RsmC can be regarded as a model system for functional streamlining of domains accompanied by the development of dependencies concerning folding and stability

Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + guanine1207 in 16S rRNA	Escherichia coli	-	S-adenosyl-L-homocysteine + N2-methylguanine1207 in 16S rRNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P39406	-	-

Purification (Commentary)

Purification (Comment)	Organism
purification and refolding of C-RsmC from inclusion bodies	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + guanine1207 in 16S rRNA	-	Escherichia coli	S-adenosyl-L-homocysteine + N2-methylguanine1207 in 16S rRNA	-	?

Synonyms

Synonyms	Comment	Organism
RsmC	-	Escherichia coli