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Literature summary for 2.1.1.17 extracted from
- A novel method for in vitro radiolabeling and testing enveloped viruses by phosphatidylethanolamine N-methyltransferase and host cell-specific binding (2006), Biotechnol. Bioeng., 94, 1017-1024.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the cell toxicity for Spodoptera frugiperda (SF-9) insect cells of the phosphatidylethanolamine N-methyltransferase preparation dissapears, when the phosphatidylethanolamine N-methyltransferase preparation is treated with 100 mg Bio-Beads SM-2 + 10 mg Butyl Toyopearl or 100 g Bio-Beads SM-2 + 10 mg CM Toyopearl.; treatment of the phosphatidylethanolamine N-methyltransferase preparation with the adsorbent Bio-Beads SM-2 decreases the phosphatidylethanolamine N-methyltransferase activity to: 24.1% with 50 mg and 28.6% with 100 mg. Addition of 1% Trition X-100 restores the activity to 90% in the experiment with 100 mg Bio-Beads SM-2.; treatment of the phosphatidylethanolamine N-methyltransferase preparation with the adsorbent Butyl Toyopearl decreases the phosphatidylethanolamine N-methyltransferase activity to: 0.2% with 10 mg; treatment of the phosphatidylethanolamine N-methyltransferase preparation with the adsorbent CM Toyopearl decreases the phosphatidylethanolamine N-methyltransferase activity to: 0.4% with 10 mg | Sus scrofa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
phosphatidylethanolamine N-methyltransferase is used to radioisotopically label the envelope of Autographa californica recombinant strain vGFPuv through the methylation of phosphatidylethanolamine. The modification of envelope phospholipids by phosphatidylethanolamine N-methyltransferase does not affect the infectivity of Autographa californica recombinant strain vGFPuv. | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Sus scrofa | - |
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