Literature summary for 2.1.1.169 extracted from

Zhou, J.M.; Seo, Y.W.; Ibrahim, R.K.
Biochemical characterization of a putative wheat caffeic acid O-methyltransferase (2008), Plant Physiol. Biochem., 47, 322-326.

Search for more literature for 2.1.1.169

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Triticum aestivum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	no requirement for Mg2+	Triticum aestivum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38900	-	x * 38900, calculated from sequence, SDS-PAGE	Triticum aestivum

Organism

Organism	UniProt	Comment	Textmining
Triticum aestivum	Q84N28	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Triticum aestivum

Storage Stability

Storage Stability	Organism
4°C, almost no loss of catalytic activity when stored for 3-4 weeks	Triticum aestivum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3 S-adenosyl-L-methionine + tricetin	TaOMT1 (TaCOMT1) catalyzes the sequential methylation of tricetin to its 3'-O-monomethyl-derivative (selgin), 3',5'-O-dimethyl-derivative (tricin) and 3',4',5'-O-trimethyl derivative. 3',5'-O-dimethyltricetin appears to be the major reaction product. The enzyme also catalyzes methylation of luteolin, quercetin and eriodictyol	Triticum aestivum	3 S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin	-	?
S-adenosyl-L-methionine + 3',5'-O-dimethyltricetin	-	Triticum aestivum	S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin	-	?
S-adenosyl-L-methionine + 3'-O-methyltricetin	-	Triticum aestivum	S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin	-	?
S-adenosyl-L-methionine + tricetin	-	Triticum aestivum	S-adenosyl-L-homocysteine + 3'-O-methyltricetin	-	?

Subunits

Subunits	Comment	Organism
?	x * 38900, calculated from sequence, SDS-PAGE	Triticum aestivum

Synonyms

Synonyms	Comment	Organism
TaCOMT1	-	Triticum aestivum
TaOMT	-	Triticum aestivum

pI Value

Organism	Comment	pI Value Maximum	pI Value
Triticum aestivum	calculated from sequence	-	5.75

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Release 2023.1 (January 2023)