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Literature summary for 2.1.1.167 extracted from

  • Lapeyre, B.; Purushothaman, S.K.
    Spb1p-directed formation of Gm2922 in the ribosome catalytic center occurs at a late processing stage (2004), Mol. Cell, 16, 663-669.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D52A when the mutant protein is the sole source of Spb1p in the cell (strain YBL4637), it leads to a severe growth defect with a generation time of about 300 min. No formation of 2'-O-methylguanosine2922 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + guanosine2922 in 27S pre-rRNA Saccharomyces cerevisiae Spb1p is a site-specific 2'-O-ribose RNA MTase that catalyzes the formation of Gm2922, a universally conserved position of the catalytic center of the ribosome that is essential for translation. The enzyme is required for proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit. Specifically methylates the guanosine in position 2922 of the 25S rRNA at the stage of 27S pre-rRNA maturation. Methylates also the uridine in position 2921 in the absence of methylation of this residue guided by snoRNA snR52 at the stage of 35S pre-rRNA maturation. 2'-O-methylguanosine2922 appears at a late processing stage, during the maturation of the 27S pre-rRNA S-adenosyl-L-homocysteine + 2'-O-methylguanosine2922 in 27S pre-rRNA
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S-adenosyl-L-methionine + uridine2918 in 25S rRNA Saccharomyces cerevisiae the enzyme is required for proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit. Specifically methylates the guanosine in position 2922 of the 25S rRNA at the stage of 27S pre-rRNA maturation. Methylates also the uridine in position 2921 in the absence of methylation of this residue guided by snoRNA snR52 at the stage of 35S pre-rRNA maturation S-adenosyl-L-homocysteine + 2'-O-methyluridine2918 in 25S rRNA
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Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + guanosine2922 in 27S pre-rRNA Spb1p is a site-specific 2'-O-ribose RNA MTase that catalyzes the formation of Gm2922, a universally conserved position of the catalytic center of the ribosome that is essential for translation. The enzyme is required for proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit. Specifically methylates the guanosine in position 2922 of the 25S rRNA at the stage of 27S pre-rRNA maturation. Methylates also the uridine in position 2921 in the absence of methylation of this residue guided by snoRNA snR52 at the stage of 35S pre-rRNA maturation. 2'-O-methylguanosine2922 appears at a late processing stage, during the maturation of the 27S pre-rRNA Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 2'-O-methylguanosine2922 in 27S pre-rRNA
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S-adenosyl-L-methionine + uridine2918 in 25S rRNA the enzyme is required for proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit. Specifically methylates the guanosine in position 2922 of the 25S rRNA at the stage of 27S pre-rRNA maturation. Methylates also the uridine in position 2921 in the absence of methylation of this residue guided by snoRNA snR52 at the stage of 35S pre-rRNA maturation Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 2'-O-methyluridine2918 in 25S rRNA
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Synonyms

Synonyms Comment Organism
Spb1p
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Saccharomyces cerevisiae