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Literature summary for 2.1.1.165 extracted from

  • Itoh, N.; Tsujita, M.; Ando, T.; Hisatomi, G.; Higashi, T.
    Formation and emission of monohalomethanes from marine algae (1997), Phytochemistry, 45, 67-73.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Iodide above 25 mM Pavlova gyrans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
S-adenosyl-L-methionine pH 7.0, 30°C Pavlova gyrans
63
-
Iodide pH 7.0, 30°C Pavlova gyrans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Papenfussiella kuromo marine microalgae are the main oceanic source of methyl bromide. The monohalomethanes produced by marine microalgae are probably important in the global cycling of gaseous organohalogen species, especially bromine and iodine ?
-
?
additional information Sargassum horneri marine microalgae are the main oceanic source of methyl bromide. The monohalomethanes produced by marine microalgae are probably important in the global cycling of gaseous organohalogen species, especially bromine and iodine ?
-
?
additional information Pavlova gyrans marine microalgae are the main oceanic source of methyl bromide. The monohalomethanes produced by marine microalgae are probably important in the global cycling of gaseous organohalogen species, especially bromine and iodine. From the viewpoint of stratospheric ozone depletion, methyl bromide is the most destructive compound because it has a high ozone depletion potential ?
-
?

Organism

Organism UniProt Comment Textmining
Papenfussiella kuromo
-
-
-
Pavlova gyrans
-
-
-
Sargassum horneri
-
-
-

Storage Stability

Storage Stability Organism
-20°C, enzyme in cell extract is unstable and loses activities almost completely upon storage even if dithioerythritol, EDTA, protease inhibitor or glycerol are added to the extracts Papenfussiella kuromo
-20°C, enzyme in cell extract is unstable and loses activities almost completely upon storage even if dithioerythritol, EDTA, protease inhibitor or glycerol are added to the extracts Sargassum horneri
4°C, enzyme in cell extract is unstable and loses activities almost completely upon storage even if dithioerythritol, EDTA, protease inhibitor or glycerol are added to the extracts Papenfussiella kuromo
4°C, enzyme in cell extract is unstable and loses activities almost completely upon storage even if dithioerythritol, EDTA, protease inhibitor or glycerol are added to the extracts Sargassum horneri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information marine microalgae are the main oceanic source of methyl bromide. The monohalomethanes produced by marine microalgae are probably important in the global cycling of gaseous organohalogen species, especially bromine and iodine Papenfussiella kuromo ?
-
?
additional information marine microalgae are the main oceanic source of methyl bromide. The monohalomethanes produced by marine microalgae are probably important in the global cycling of gaseous organohalogen species, especially bromine and iodine Sargassum horneri ?
-
?
additional information marine microalgae are the main oceanic source of methyl bromide. The monohalomethanes produced by marine microalgae are probably important in the global cycling of gaseous organohalogen species, especially bromine and iodine. From the viewpoint of stratospheric ozone depletion, methyl bromide is the most destructive compound because it has a high ozone depletion potential Pavlova gyrans ?
-
?
additional information no activity with chloride, no activity with L-methionine, S-methyl methionine or dimethylsulfoniopropionate Pavlova gyrans ?
-
?
S-adenosyl-L-methionine + bromide
-
Pavlova gyrans S-adenosyl-L-homocysteine + methyl bromide
-
?
S-adenosyl-L-methionine + iodide
-
Pavlova gyrans S-adenosyl-L-homocysteine + methyl iodide
-
?
S-adenosyl-L-methionine + iodide
-
Papenfussiella kuromo S-adenosyl-L-homocysteine + methyl iodide
-
?
S-adenosyl-L-methionine + iodide
-
Sargassum horneri S-adenosyl-L-homocysteine + methyl iodide
-
?

Synonyms

Synonyms Comment Organism
SAM:halide ion methyltransferase
-
Pavlova gyrans
SAM:halide ion methyltransferase
-
Papenfussiella kuromo
SAM:halide ion methyltransferase
-
Sargassum horneri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Pavlova gyrans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
Pavlova gyrans

pH Range

pH Minimum pH Maximum Comment Organism
6 8 about 60% of maximal activity at pH 6.0 and 8.0 Pavlova gyrans