Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bisulfide | - |
Brassica oleracea | |
S-adenosyl-L-homocysteine | competitive with respect to S-adenosyl-L-methionine, noncompetitive to iodide | Brassica oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
S-adenosyl-L-methionine | pH 7.5, 22°C, cosubstrate: iodide | Brassica oleracea | |
1.3 | - |
Iodide | pH 7.5, 22°C | Brassica oleracea | |
29 | - |
bromide | pH 7.5, 22°C | Brassica oleracea | |
85 | - |
chloride | pH 7.5, 22°C | Brassica oleracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
1 * 28000, SDS-PAFE | Brassica oleracea |
29500 | - |
gel filtration | Brassica oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration, anion exchange chromatography, and affinity chromatography on adenosine-agarose | Brassica oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Brassica oleracea | - |
Storage Stability | Organism |
---|---|
- 80°C, enzyme after the first gel filtration purification step, in 25 mM Tris acetate, pH 7.4, 10% glycerol, and 14 mM 2-mercaptoethanol, stable for over 2 months | Brassica oleracea |
-80°C, after affinity chromatography, the halide/bisulfide methyltransferase becomes extremely labile losing all activity after overnight storage | Brassica oleracea |
20°C, enzyme after the affinity chromatography purification step, 25 mM Tris acetate, pH 7.4, 14 mM 2-mercaptoethanol, and 30% glycerol, 12% remaining activity after 48 h | Brassica oleracea |
4°C, enzyme after anion exchange purification step, in 25 mM Tris acetate, pH 7.4, 14 mM 2-mercaptoethanol, and 175 mM NaCl, more than 70% remaining activity after 24 h and 55% after 48 h | Brassica oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the bifunctional enzyme also shows activity of EC 2.1.1.9 | Brassica oleracea | ? | - |
? | |
S-adenosyl-L-methionine + bromide | kcat/KM for bromide is 12529fold lower than kcat/Km for iodide | Brassica oleracea | S-adenosyl-L-homocysteine + methyl bromide | - |
? | |
S-adenosyl-L-methionine + chloride | kcat/KM for chloride is 19065fold lower than kcat/Km for iodide | Brassica oleracea | S-adenosyl-L-homocysteine + methyl chloride | - |
? | |
S-adenosyl-L-methionine + iodide | iodide is the preferred substrate | Brassica oleracea | S-adenosyl-L-homocysteine + methyl iodide | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 28000, SDS-PAFE | Brassica oleracea |
Synonyms | Comment | Organism |
---|---|---|
halide/bisulfide methyltransferase | bifunctional enzyme also shows activity of EC 2.1.1.9 | Brassica oleracea |
S-adenosyl-L-methionine:halide/bisulfide methyltransferase | bifunctional enzyme also shows activity of EC 2.1.1.9 | Brassica oleracea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Brassica oleracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7 | methylation of iodide | Brassica oleracea |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
S-adenosyl-L-homocysteine | pH 7.5, 22°C | Brassica oleracea | |
1.35 | - |
bisulfide | pH 7.5, 22°C | Brassica oleracea |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Brassica oleracea | chromatofocusing | - |
4.8 |