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Literature summary for 2.1.1.165 extracted from

  • Attieh, J.M.; Hanson, A.D.; Saini, H.S.
    Purification and characterization of a novel methyltransferase responsible for biosynthesis of halomethanes and methanethiol in Brassica oleracea (1995), J. Biol. Chem., 270, 9250-9257.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
bisulfide
-
Brassica oleracea
S-adenosyl-L-homocysteine competitive with respect to S-adenosyl-L-methionine, noncompetitive to iodide Brassica oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
S-adenosyl-L-methionine pH 7.5, 22°C, cosubstrate: iodide Brassica oleracea
1.3
-
Iodide pH 7.5, 22°C Brassica oleracea
29
-
bromide pH 7.5, 22°C Brassica oleracea
85
-
chloride pH 7.5, 22°C Brassica oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
1 * 28000, SDS-PAFE Brassica oleracea
29500
-
gel filtration Brassica oleracea

Organism

Organism UniProt Comment Textmining
Brassica oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration, anion exchange chromatography, and affinity chromatography on adenosine-agarose Brassica oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Brassica oleracea
-

Storage Stability

Storage Stability Organism
- 80°C, enzyme after the first gel filtration purification step, in 25 mM Tris acetate, pH 7.4, 10% glycerol, and 14 mM 2-mercaptoethanol, stable for over 2 months Brassica oleracea
-80°C, after affinity chromatography, the halide/bisulfide methyltransferase becomes extremely labile losing all activity after overnight storage Brassica oleracea
20°C, enzyme after the affinity chromatography purification step, 25 mM Tris acetate, pH 7.4, 14 mM 2-mercaptoethanol, and 30% glycerol, 12% remaining activity after 48 h Brassica oleracea
4°C, enzyme after anion exchange purification step, in 25 mM Tris acetate, pH 7.4, 14 mM 2-mercaptoethanol, and 175 mM NaCl, more than 70% remaining activity after 24 h and 55% after 48 h Brassica oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the bifunctional enzyme also shows activity of EC 2.1.1.9 Brassica oleracea ?
-
?
S-adenosyl-L-methionine + bromide kcat/KM for bromide is 12529fold lower than kcat/Km for iodide Brassica oleracea S-adenosyl-L-homocysteine + methyl bromide
-
?
S-adenosyl-L-methionine + chloride kcat/KM for chloride is 19065fold lower than kcat/Km for iodide Brassica oleracea S-adenosyl-L-homocysteine + methyl chloride
-
?
S-adenosyl-L-methionine + iodide iodide is the preferred substrate Brassica oleracea S-adenosyl-L-homocysteine + methyl iodide
-
?

Subunits

Subunits Comment Organism
monomer 1 * 28000, SDS-PAFE Brassica oleracea

Synonyms

Synonyms Comment Organism
halide/bisulfide methyltransferase bifunctional enzyme also shows activity of EC 2.1.1.9 Brassica oleracea
S-adenosyl-L-methionine:halide/bisulfide methyltransferase bifunctional enzyme also shows activity of EC 2.1.1.9 Brassica oleracea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at Brassica oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7 methylation of iodide Brassica oleracea

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.032
-
S-adenosyl-L-homocysteine pH 7.5, 22°C Brassica oleracea
1.35
-
bisulfide pH 7.5, 22°C Brassica oleracea

pI Value

Organism Comment pI Value Maximum pI Value
Brassica oleracea chromatofocusing
-
4.8