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Literature summary for 2.1.1.164 extracted from

  • Zhang, C.; Albermann, C.; Fu, X.; Peters, N.R.; Chisholm, J.D.; Zhang, G.; Gilbert, E.J.; Wang, P.G.; van Vranken, D.L.; Thorson, J.S.
    RebG- and RebM-catalyzed indolocarbazole diversification (2006), Chembiochem, 7, 795-804.
    View publication on PubMed
Search for more literature for 2.1.1.164

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 1 mM, 8% inhibition Lentzea aerocolonigenes
Co2+ 1 mM, 87% inhibition Lentzea aerocolonigenes
Cu2+ 1 mM, 93% inhibition Lentzea aerocolonigenes
EDTA 1 mM, 5% inhibition Lentzea aerocolonigenes
Fe2+ 1 mM, 17% inhibition Lentzea aerocolonigenes
Mg2+ 1 mM, 3% inhibition Lentzea aerocolonigenes
Mn2+ 1 mM, 48% inhibition Lentzea aerocolonigenes
Ni2+ 1 mM, complete inhibition Lentzea aerocolonigenes
Zn2+ 1 mM, complete inhibition Lentzea aerocolonigenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0021
-
 dechlorinated 4'-demethyl-rebeccamycin pH 8.0, 30°C Lentzea aerocolonigenes
0.012
-
 S-adenosyl-L-methionine pH 8.0, 30°C, cosubstrate: dechlorinated 4'-demethyl-rebeccamycin Lentzea aerocolonigenes

Metals/Ions

Metals/Ions Comment Organism Structure
additional information RebM activity is not enhanced by divalent metals Lentzea aerocolonigenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31400
-
 1 * 31400, calculated from sequence Lentzea aerocolonigenes
32000
-
 1 * 32000, SDS-PAGE Lentzea aerocolonigenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4'-demethylrebeccamycin + S-adenosyl-L-methionine Lentzea aerocolonigenes last step in the biosynthesis of rebeccamycin rebeccamycin + S-adenosyl-L-homocysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Lentzea aerocolonigenes Q8KZ94
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-bromo-11-beta-D-glucopyranosyl-11,12-dihydroindolo[2,3-a]carbazole + S-adenosyl-L-methionine
-
 Lentzea aerocolonigenes ? + S-adenosyl-L-homocysteine
-
 ?
3-bromo-12-beta-D-glucopyranosyl-11,12-dihydroindolo[2,3-a]carbazole + S-adenosyl-L-methionine
-
 Lentzea aerocolonigenes ? + S-adenosyl-L-homocysteine
-
 ?
4'-demethylrebeccamycin + S-adenosyl-L-methionine
-
 Lentzea aerocolonigenes rebeccamycin + S-adenosyl-L-homocysteine
-
 ?
4'-demethylrebeccamycin + S-adenosyl-L-methionine last step in the biosynthesis of rebeccamycin Lentzea aerocolonigenes rebeccamycin + S-adenosyl-L-homocysteine
-
 ?
dechlorinated 4'-demethyl-rebeccamycin + S-adenosyl-L-methionine
-
 Lentzea aerocolonigenes dechlorinated rebeccamycin + S-adenosyl-L-homocysteine
-
 ?
dechlorinated 5-deoxo-4'-demethyl-rebeccamycin + S-adenosyl-L-methionine
-
 Lentzea aerocolonigenes ? + S-adenosyl-L-homocysteine
-
 ?
dechlorinated 6-methyl-2'-deoxy-4'-demethyl-alpha-D-Glc-rebeccamycin + S-adenosyl-L-methionine
-
 Lentzea aerocolonigenes ? + S-adenosyl-L-homocysteine
-
 ?
dechlorinated 6-methyl-2'-deoxy-4'-demethyl-rebeccamycin + S-adenosyl-L-methionine
-
 Lentzea aerocolonigenes ? + S-adenosyl-L-homocysteine
-
 ?
dechlorinated 6-[3-(1H-imidazol-1-yl)propyl]-4'-demethyl-rebeccamycin + S-adenosyl-L-methionine
-
 Lentzea aerocolonigenes ? + S-adenosyl-L-homocysteine
-
 ?
dechlorinated 7-deoxo-4'-demethyl-rebeccamycin + S-adenosyl-L-methionine
-
 Lentzea aerocolonigenes ? + S-adenosyl-L-homocysteine
-
 ?
additional information RebM accepts a wide range of alternate substrates. Specifically, variation on the imide heterocycle by removal or addition of a bulky group is tolerated by RebM. Deoxygenation of the sugar moiety only slightly decreases RebM activity. RebM displays flexibility toward anomers and is able to process both alpha and beta-glycosidic analogues Lentzea aerocolonigenes ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 32000, SDS-PAGE Lentzea aerocolonigenes
monomer 1 * 31400, calculated from sequence Lentzea aerocolonigenes

Synonyms

Synonyms Comment Organism
RebM
-
 Lentzea aerocolonigenes

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.047
-
 dechlorinated 4'-demethyl-rebeccamycin pH 8.0, 30°C Lentzea aerocolonigenes
0.047
-
 S-adenosyl-L-methionine pH 8.0, 30°C, cosubstrate: dechlorinated 4'-demethyl-rebeccamycin Lentzea aerocolonigenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 8 functional over a broader pH range from pH 6.5 to above 8.0 Lentzea aerocolonigenes

Expression

Organism Comment Expression
Lentzea aerocolonigenes RebM expression is induced by the addition of isopropyl-beta-D-thiogalactopyranoside (0.4 mm final concentration) up