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Literature summary for 2.1.1.163 extracted from

  • Koike-Takeshita, A.; Koyama, T.; Ogura, K.
    Identification of a novel gene cluster participating in menaquinone (vitamin K2) biosynthesis. Cloning and sequence determination of the 2-heptaprenyl-1,4-naphthoquinone methyltransferase gene of Bacillus stearothermophilus (1997), J. Biol. Chem., 272, 12380-12383.
    View publication on PubMed
Search for more literature for 2.1.1.163

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Geobacillus stearothermophilus
gene heps-2, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain JM109 Geobacillus stearothermophilus

Protein Variants

Protein Variants Comment Organism
additional information expression in Escherichia coli complements mutation AN70 deficient of a specific methyltransferase in the ubiquinone biosynthetic pathway Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus O86169
-
-
Geobacillus stearothermophilus O86169 gene heps-2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-heptaprenyl-6-methoxy-1,4-benzoquinone + S-adenosyl-L-methionine
-
 Geobacillus stearothermophilus 2-heptaprenyl-6-methoxy-3-methyl-1,4-benzoquinone + S-adenosyl-L-homocysteine
-
 ?

Synonyms

Synonyms Comment Organism
2-heptaprenyl-1,4-naphthoquinone methyltransferase
-
 Geobacillus stearothermophilus
menaquinone biosynthesis methyltransferase
-
 Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine dependent on Geobacillus stearothermophilus

General Information

General Information Comment Organism
evolution ORF-1, ORF-2, and ORF-3 genes, designated heps-1, menG, and heps-2, respectively, form another cluster involved in menaquinone biosynthesis in addition to the cluster of menB, menC, menD, and menE already identified in the Bacillus subtilis and Escherichia coli chromosomes Geobacillus stearothermophilus
metabolism the demethylmenaquinone methyltransferase or 2-heptaprenyl-1,4-naphthoquinone synthase participates in the terminal step of the menaquinone biosynthesis Geobacillus stearothermophilus