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Literature summary for 2.1.1.162 extracted from

  • Lai, M.C.; Wang, C.C.; Chuang, M.J.; Wu, Y.C.; Lee, Y.C.
    Effects of substrate and potassium on the betaine-synthesizing enzyme glycine sarcosine dimethylglycine N-methyltransferase from a halophilic methanoarchaeon Methanohalophilus portucalensis (2006), Res. Microbiol., 157, 948-955.
    View publication on PubMed
Search for more literature for 2.1.1.162

Inhibitors

Inhibitors Comment Organism Structure
S-adenosyl-L-homocysteine complete inhibition above 0.6 mM Methanohalophilus portucalensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 glycine pH 7.2, 37°C Methanohalophilus portucalensis
2.45
-
 N,N-dimethylglycine pH 7.2, 37°C Methanohalophilus portucalensis
3.14
-
 N-methylglycine pH 7.2, 37°C Methanohalophilus portucalensis

Metals/Ions

Metals/Ions Comment Organism Structure
K+ two different proteins eluted from chromatofocusing column are differently activated by addition of K+, the peak D protein exhibits low GSDMT activity only at K+ Methanohalophilus portucalensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 4 * 52000, SDS-PAGE, native mass of 240000 Da by native PAGE, different pI detected for the individual subunits Methanohalophilus portucalensis
240000
-
 native PAGE Methanohalophilus portucalensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + glycine Methanohalophilus portucalensis synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine S-adenosyl-L-homocysteine + N-methylglycine
-
 ?
S-adenosyl-L-methionine + N,N-dimethylglycine Methanohalophilus portucalensis synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
-
 ?
S-adenosyl-L-methionine + N-methylglycine Methanohalophilus portucalensis synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
 ?

Organism

Organism UniProt Comment Textmining
Methanohalophilus portucalensis
-
-
-
Methanohalophilus portucalensis
-
 two different peaks showing GSDMT activity eluted from chromatofocusing column
-
Methanohalophilus portucalensis FDF1
-
-
-
Methanohalophilus portucalensis FDF1
-
 two different peaks showing GSDMT activity eluted from chromatofocusing column
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanohalophilus portucalensis

Reaction

Reaction Comment Organism Reaction ID
3 S-adenosyl-L-methionine + glycine = 3 S-adenosyl-L-homocysteine + betaine (overall reaction) Methanohalophilus portucalensis
a
S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + sarcosine (1a) Methanohalophilus portucalensis
a
S-adenosyl-L-methionine + N,N-dimethylglycine = S-adenosyl-L-homocysteine + betaine + H+ (1c) Methanohalophilus portucalensis
a
S-adenosyl-L-methionine + sarcosine = S-adenosyl-L-homocysteine + N,N-dimethylglycine (1b) Methanohalophilus portucalensis
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.003
-
 N,N-dimethylglycine as substrate, pH 7.2, 37°C Methanohalophilus portucalensis
0.0037
-
 glycine as substrate, pH 7.2, 37°C Methanohalophilus portucalensis
0.0046
-
 N-methylglycine as substrate, pH 7.2, 37°C Methanohalophilus portucalensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 S-adenosyl-L-methionine + glycine (overall reaction) Methanohalophilus portucalensis 3 S-adenosyl-L-homocysteine + betaine
-
 ?
S-adenosyl-L-methionine + glycine (1a) Methanohalophilus portucalensis S-adenosyl-L-homocysteine + sarcosine
-
 ?
S-adenosyl-L-methionine + glycine (1a) Methanohalophilus portucalensis FDF1 S-adenosyl-L-homocysteine + sarcosine
-
 ?
S-adenosyl-L-methionine + glycine synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine Methanohalophilus portucalensis S-adenosyl-L-homocysteine + N-methylglycine
-
 ?
S-adenosyl-L-methionine + N,N-dimethylglycine (1c) Methanohalophilus portucalensis S-adenosyl-L-homocysteine + betaine
-
 ?
S-adenosyl-L-methionine + N,N-dimethylglycine (1c) Methanohalophilus portucalensis FDF1 S-adenosyl-L-homocysteine + betaine
-
 ?
S-adenosyl-L-methionine + N,N-dimethylglycine synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine Methanohalophilus portucalensis S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
-
 ?
S-adenosyl-L-methionine + N-methylglycine synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine Methanohalophilus portucalensis S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
 ?
S-adenosyl-L-methionine + sarcosine (1b) Methanohalophilus portucalensis S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
 ?
S-adenosyl-L-methionine + sarcosine (1b) Methanohalophilus portucalensis FDF1 S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 52000, SDS-PAGE, native mass of 240000 Da by native PAGE, different pI detected for the individual subunits Methanohalophilus portucalensis

Synonyms

Synonyms Comment Organism
GSDMT
-
 Methanohalophilus portucalensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 glycine as subtrate Methanohalophilus portucalensis

pI Value

Organism Comment pI Value Maximum pI Value
Methanohalophilus portucalensis alpha-subunit, 2-D gel electrophoresis
-
 4.4
Methanohalophilus portucalensis beta-subunit, 2-D gel electrophoresis
-
 4.8
Methanohalophilus portucalensis gamma-subunit, 2-D gel electrophoresis
-
 5
Methanohalophilus portucalensis delta-subunit, 2-D gel electrophoresis
-
 5.2