Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3), sequence comparisonof caffeine synthetic enzymes from coffee | Coffea sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of several enzyme mutants, overview | Coffea sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 1,7-dimethylxanthine | Coffea sp. | - |
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | - |
? | |
S-adenosyl-L-methionine + 3,7-dimethylxanthine | Coffea sp. | - |
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | - |
? | |
S-adenosyl-L-methionine + 7-methylxanthine | Coffea sp. | - |
S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coffea sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the descending order of activity of the purine base with caffeine synthase is N-3, N-1, N-7 | Coffea sp. | ? | - |
? | |
S-adenosyl-L-methionine + 1,7-dimethylxanthine | - |
Coffea sp. | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | - |
? | |
S-adenosyl-L-methionine + 1,7-dimethylxanthine | paraxanthine is the best methyl acceptor for caffeine synthase | Coffea sp. | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | - |
? | |
S-adenosyl-L-methionine + 3,7-dimethylxanthine | - |
Coffea sp. | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | - |
? | |
S-adenosyl-L-methionine + 7-methylxanthine | - |
Coffea sp. | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CCS1 | - |
Coffea sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Coffea sp. |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the second and third step in caffeine biosynthesis, pathway overview | Coffea sp. |