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Literature summary for 2.1.1.160 extracted from

  • Mizuno, K.; Okuda, A.; Kato, M.; Yoneyama, N.; Tanaka, H.; Ashihara, H.; Fujimura, T.
    Isolation of a new dual-functional caffeine synthase gene encoding an enzyme for the conversion of 7-methylxanthine to caffeine from coffee (Coffea arabica L.) (2003), FEBS Lett., 534, 75-81.
    View publication on PubMed
Search for more literature for 2.1.1.160

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression in Escherichia coli as wild-type and as Trx-fusion protein Coffea arabica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.157
-
 Theobromine pH 8.5, 37°C, recombinant CCS1 Coffea arabica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Coffea arabica bifunctional enzyme performing 3-N-methyltransferase activity, EC 2.1.1.159, and 1-N-methyltransferase activity, EC 2.1.1.160, overview ?
-
 ?
S-adenosyl-L-methionine + 3,7-dimethylxanthine Coffea arabica last step in caffeine biosynthesis pathway S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?

Organism

Organism UniProt Comment Textmining
Coffea arabica Q8H0D3 bifunctional enzyme performing 3-N-methyltransferase activity, EC 2.1.1.159, and 1-N-methyltransferase activity, EC 2.1.1.160; isozyme CtCS6 is a bifunctional enzyme
-

Source Tissue

Source Tissue Comment Organism Textmining
endosperm developing Coffea arabica
-
flower bud
-
 Coffea arabica
-
leaf young Coffea arabica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information bifunctional enzyme performing 3-N-methyltransferase activity, EC 2.1.1.159, and 1-N-methyltransferase activity, EC 2.1.1.160, overview Coffea arabica ?
-
 ?
additional information substrate specificity, bifunctional enzyme performing 3-N-methyltransferase activity, EC 2.1.1.159, and 1-N-methyltransferase activity, EC 2.1.1.160, overview Coffea arabica ?
-
 ?
S-adenosyl-L-methionine + 3,7-dimethylxanthine
-
 Coffea arabica S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
S-adenosyl-L-methionine + 3,7-dimethylxanthine last step in caffeine biosynthesis pathway Coffea arabica S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?

Synonyms

Synonyms Comment Organism
1-N-methyltransferase
-
 Coffea arabica
caffeine synthase 1
-
 Coffea arabica
CCS1
-
 Coffea arabica
coffee caffeine synthase 1
-
 Coffea arabica
CtCS6
-
 Coffea arabica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Coffea arabica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Coffea arabica