Cloned (Comment) | Organism |
---|---|
cloning of MXMT vectors for Agrobacterium tumefaciens-mediated transformation | Coffea canephora |
DNA and amino acid sequence determination and analysis, cloning of MXMT vectors for Agrobacterium tumefaciens-mediated transformation | Coffea arabica |
gene MXMT2, DNA and amino acid sequence determination and analysis | Coffea arabica |
Protein Variants | Comment | Organism |
---|---|---|
additional information | suppression of enzyme expression by RNA interference method, method development for somatic embryogenesis, Agrobacterium tumefaciens-mediated transformation, the transgenic plants show reduced caffeine content, overview | Coffea canephora |
additional information | suppression of enzyme expression by RNA interference method, method development for somatic embryogenesis, Agrobacterium tumefaciens-mediated transformation, the transgenic plants show reduced caffeine content, overview | Coffea arabica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 7-methylxanthine | Coffea arabica | second step in caffeine biosynthesis pathway, theobromine is the major intermediate for caffeine biosynthesis | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir | |
S-adenosyl-L-methionine + 7-methylxanthine | Coffea canephora | second step in caffeine biosynthesis pathway, theobromine is the major intermediate for caffeine biosynthesis, overview | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coffea arabica | Q84PP7 | gene CaMXMT2, isozyme MXMT2; var. caturra, isozyme MXMT2 | - |
Coffea arabica | Q9AVJ9 | gene CaMXMT1, isozyme MXMT1; var. caturra, isozyme MXMT1 | - |
Coffea canephora | - |
var. conilon | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | young | Coffea canephora | - |
leaf | young | Coffea arabica | - |
additional information | method development for somatic embryogenesis, overview | Coffea canephora | - |
additional information | method development for somatic embryogenesis, overview | Coffea arabica | - |
seedling | derived from somatic embryogenesis | Coffea canephora | - |
seedling | derived from somatic embryogenesis | Coffea arabica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
determination of in vivo activity in wild-type and mutant plants by determination of theobromine concentration in seedlings and young leaves | Coffea canephora |
additional information | - |
determination of in vivo activity in wild-type and mutant plants by determination of theobromine concentration in seedlings and young leaves | Coffea arabica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 7-methylxanthine | - |
Coffea canephora | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir | |
S-adenosyl-L-methionine + 7-methylxanthine | - |
Coffea arabica | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir | |
S-adenosyl-L-methionine + 7-methylxanthine | second step in caffeine biosynthesis pathway, theobromine is the major intermediate for caffeine biosynthesis | Coffea arabica | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir | |
S-adenosyl-L-methionine + 7-methylxanthine | second step in caffeine biosynthesis pathway, theobromine is the major intermediate for caffeine biosynthesis, overview | Coffea canephora | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir |
Synonyms | Comment | Organism |
---|---|---|
7-methylxanthine methyltransferase | - |
Coffea arabica |
MXMT | - |
Coffea canephora |
MXMT1 | - |
Coffea arabica |
MXMT2 | - |
Coffea arabica |