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Literature summary for 2.1.1.159 extracted from

  • Ogita, S.; Uefuji, H.; Morimoto, M.; Sano, H.
    Application of RNAi to confirm theobromine as the major intermediate for caffeine biosynthesis in coffee plants with potential for construction of decaffeinated varieties (2004), Plant Mol. Biol., 54, 931-941.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloning of MXMT vectors for Agrobacterium tumefaciens-mediated transformation Coffea canephora
DNA and amino acid sequence determination and analysis, cloning of MXMT vectors for Agrobacterium tumefaciens-mediated transformation Coffea arabica
gene MXMT2, DNA and amino acid sequence determination and analysis Coffea arabica

Protein Variants

Protein Variants Comment Organism
additional information suppression of enzyme expression by RNA interference method, method development for somatic embryogenesis, Agrobacterium tumefaciens-mediated transformation, the transgenic plants show reduced caffeine content, overview Coffea canephora
additional information suppression of enzyme expression by RNA interference method, method development for somatic embryogenesis, Agrobacterium tumefaciens-mediated transformation, the transgenic plants show reduced caffeine content, overview Coffea arabica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 7-methylxanthine Coffea arabica second step in caffeine biosynthesis pathway, theobromine is the major intermediate for caffeine biosynthesis S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ir
S-adenosyl-L-methionine + 7-methylxanthine Coffea canephora second step in caffeine biosynthesis pathway, theobromine is the major intermediate for caffeine biosynthesis, overview S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ir

Organism

Organism UniProt Comment Textmining
Coffea arabica Q84PP7 gene CaMXMT2, isozyme MXMT2; var. caturra, isozyme MXMT2
-
Coffea arabica Q9AVJ9 gene CaMXMT1, isozyme MXMT1; var. caturra, isozyme MXMT1
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Coffea canephora
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var. conilon
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Source Tissue

Source Tissue Comment Organism Textmining
leaf young Coffea canephora
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leaf young Coffea arabica
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additional information method development for somatic embryogenesis, overview Coffea canephora
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additional information method development for somatic embryogenesis, overview Coffea arabica
-
seedling derived from somatic embryogenesis Coffea canephora
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seedling derived from somatic embryogenesis Coffea arabica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
determination of in vivo activity in wild-type and mutant plants by determination of theobromine concentration in seedlings and young leaves Coffea canephora
additional information
-
determination of in vivo activity in wild-type and mutant plants by determination of theobromine concentration in seedlings and young leaves Coffea arabica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 7-methylxanthine
-
Coffea canephora S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ir
S-adenosyl-L-methionine + 7-methylxanthine
-
Coffea arabica S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ir
S-adenosyl-L-methionine + 7-methylxanthine second step in caffeine biosynthesis pathway, theobromine is the major intermediate for caffeine biosynthesis Coffea arabica S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ir
S-adenosyl-L-methionine + 7-methylxanthine second step in caffeine biosynthesis pathway, theobromine is the major intermediate for caffeine biosynthesis, overview Coffea canephora S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ir

Synonyms

Synonyms Comment Organism
7-methylxanthine methyltransferase
-
Coffea arabica
MXMT
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Coffea canephora
MXMT1
-
Coffea arabica
MXMT2
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Coffea arabica