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Literature summary for 2.1.1.158 extracted from
- Molecular cloning and functional characterization of three distinct N-methyltransferases involved in the caffeine biosynthetic pathway in coffee plants (2003), Plant Physiol., 132, 372-380.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| isozyme XMT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of the GST-tagged enzyme in Escherichia coli Bl21(DE3) | Coffea arabica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.078 | - |
Xanthosine | recombinant XMT1 | Coffea arabica |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41800 | - |
2 * 41800, recombinant isozyme XMT1, SDS-PAGE | Coffea arabica |
| 69000 | - |
recombinant isozyme XMT1, gel filtration | Coffea arabica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + xanthosine | Coffea arabica | - |
S-adenosyl-L-homocysteine + 7-methylxanthosine | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Coffea arabica | Q9AVK0 | isozyme XMT1; isozyme XMT1 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower bud | high expression | Coffea arabica | - |
| fruit | high expression level in immature fruits, low expression in mature fruits | Coffea arabica | - |
| leaf | very high expression in young leaves, moderate expression in expanded leaves | Coffea arabica | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
recombinant enzyme | Coffea arabica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + xanthosine | - |
Coffea arabica | S-adenosyl-L-homocysteine + 7-methylxanthosine | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 41800, recombinant isozyme XMT1, SDS-PAGE | Coffea arabica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| XMT1 | - |
Coffea arabica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 27 | - |
assay at | Coffea arabica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Coffea arabica |
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Release 2023.1 (January 2023)
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