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Literature summary for 2.1.1.153 extracted from

  • Knogge, W.; Weissenboeck, G.
    Purification, characterization, and kinetic mechanism of S-adenosyl-L-methionine: vitexin 2"-O-rhamnoside 7-O-methyltransferase of Avena sativa L (1984), Eur. J. Biochem., 140, 113-118.
    View publication on PubMed

General Stability

General Stability Organism
2-mercaptoethanol stabilizes during purification procedures or storage Avena sativa

Inhibitors

Inhibitors Comment Organism Structure
7-O-methylvitexin competitive inhibition Avena sativa
7-O-methylvitexin 2''-O-beta-L-rhamnoside product inhibition, kinetics Avena sativa
additional information not inhibited by Mg2+ or 2-mercaptoethanol Avena sativa
S-adenosyl-L-homocysteine strong product inhibition, kinetics Avena sativa
vitexin competitive inhibition Avena sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic mechanism, mono-iso Theorell-Chance mechanism with the nucleotide substrate binding before the flavonoid Avena sativa
0.0016
-
S-adenosyl-L-methionine pH 7.5, 30°C Avena sativa
0.015
-
vitexin 2''-O-beta-L-rhamnoside pH 7.5, 30°C Avena sativa

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not activated by Mg2+ or 2-mercaptoethanol Avena sativa
phosphate the highest activity is obtained in 0.1-0.2 M phosphate buffer, at lower ionic strength the activity drops drastically Avena sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
gel filtration Avena sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + vitexin 2''-O-beta-L-rhamnoside Avena sativa last step in the biosynthetic pathway to the flavonoid 7-O-methylvitexin 2’’-O-rhamnoside S-adenosyl-L-homocysteine + 7-O-methylvitexin 2''-O-beta-L-rhamnoside
-
?

Organism

Organism UniProt Comment Textmining
Avena sativa
-
Gelbhafer Flämingskrone
-

Purification (Commentary)

Purification (Comment) Organism
180fold Avena sativa

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + vitexin 2''-O-beta-L-rhamnoside = S-adenosyl-L-homocysteine + 7-O-methylvitexin 2''-O-beta-L-rhamnoside + H+ the flavonoids vitexin and isovitexin 2’’-O-arabinoside do not act as substrates for the enzyme from oats, Avena sativa, kinetic mechanism, mono-iso Theorell-Chance mechanism Avena sativa

Source Tissue

Source Tissue Comment Organism Textmining
leaf primary leaves Avena sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Avena sativa

Storage Stability

Storage Stability Organism
-20°C, purified enzyme, 10 mM 2-mercaptoethanol, 50% glycerol, 1 year, 30-40% loss of activity Avena sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information not: caffeic acid, naringenin, vitexin, isovitexin 2’’-O-arabinoside Avena sativa ?
-
?
S-adenosyl-L-methionine + apigenin 5-10% of the rate with vitexin 2’’-O-rhamnoside as substrate Avena sativa S-adenosyl-L-homocysteine + 7-O-methylapigenin
-
?
S-adenosyl-L-methionine + vitexin 2''-O-beta-L-rhamnoside last step in the biosynthetic pathway to the flavonoid 7-O-methylvitexin 2’’-O-rhamnoside Avena sativa S-adenosyl-L-homocysteine + 7-O-methylvitexin 2''-O-beta-L-rhamnoside
-
?
S-adenosyl-L-methionine + vitexin 2''-O-beta-L-rhamnoside catalyzes the transfer of the methyl group of S-adenosyl-L-methionine to the A-ring 7-hydroxyl group of vitexin 2’’-O-rhamnoside, very high substrate specificity, mono-iso Theorell-Chance mechanism with the nucleotide substrate binding before the flavonoid Avena sativa S-adenosyl-L-homocysteine + 7-O-methylvitexin 2''-O-beta-L-rhamnoside
-
?

Synonyms

Synonyms Comment Organism
AdoMet:vitexin 2''-O-rhamnoside 7-O-methyltransferase
-
Avena sativa
methyltransferase, vitexin 2''-O-rhamnoside 7-O-
-
Avena sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Avena sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Avena sativa

pH Range

pH Minimum pH Maximum Comment Organism
6.4 8.6 50% of the maximum activity at pH 6.4 and 8.6 Avena sativa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information
-
Avena sativa