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Literature summary for 2.1.1.150 extracted from
- Stress responses in alfalfa (Medicago sativa L). XXII. cDNA cloning and characterization of an elicitor-inducible isoflavone 7-O-methyltransferase (1998), Plant Mol. Biol., 36, 43-54.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Medicago sativa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| up | IOMT transcripts are rapidly induced following elicitation, prior to the increase in IOMT activity | Medicago sativa |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39603 | - |
x * 39603, calculated from sequence | Medicago sativa |
| 41000 | - |
x * 41000, SDS-PAGE | Medicago sativa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone | Medicago sativa | i.e. daidzein | S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone | i.e. isoformononetin | ? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Medicago sativa | O24529 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| callus | - |
Medicago sativa | - |
| cell suspension culture | - |
Medicago sativa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + 5,7,4'-trihydroxyisoflavone | i.e. genistein. 12.9% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein) | Medicago sativa | S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyisoflavone | - |
? | |
| S-adenosyl-L-methionine + 6,7,4'-trihydroxyisoflavone | 136% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein) | Medicago sativa | S-adenosyl-L-homocysteine + 6,4'-dihydroxy-7-methoxyisoflavone | - |
? | |
| S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone | i.e. daidzein | Medicago sativa | S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone | i.e. isoformononetin | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 41000, SDS-PAGE | Medicago sativa |
| ? | x * 39603, calculated from sequence | Medicago sativa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 7-IOMT | - |
Medicago sativa |
| IOMT8 | - |
Medicago sativa |
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Release 2023.1 (January 2023)
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