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Literature summary for 2.1.1.15 extracted from
- Engineering Escherichia coli for biodiesel production utilizing a bacterial fatty acid methyltransferase (2011), Appl. Environ. Microbiol., 77, 8052-8061.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3)(pLysS) | Mycobacterium marinum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.046 | - |
Decanoic acid | pH 7.8, 37°C | Mycobacterium marinum |  |
| 0.077 | - |
octanoic acid | pH 7.8, 37°C | Mycobacterium marinum | |
| 0.099 | - |
3-hydroxydecanoic acid | pH 7.8, 37°C | Mycobacterium marinum | |
| 0.103 | - |
dodecanoic acid | pH 7.8, 37°C | Mycobacterium marinum | |
| 0.129 | - |
3-hydroxydodecanoic acid | pH 7.8, 37°C | Mycobacterium marinum | |
| 0.196 | - |
3-hydroxyoctanoic acid | pH 7.8, 37°C | Mycobacterium marinum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium marinum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| immobilized metal ion affinity chromatography (Ni2+) | Mycobacterium marinum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-hydroxydecanoic acid + S-adenosyl-L-methionine | - |
Mycobacterium marinum | methyl 3-hydroxydecanoate + S-adenosyl-L-homocysteine | - |
? | |
| 3-hydroxydodecanoic acid + S-adenosyl-L-methionine | - |
Mycobacterium marinum | methyl 3-hydroxydodecanoate + S-adenosyl-L-homocysteine | - |
? | |
| 3-hydroxyoctanoic acid + S-adenosyl-L-methionine | - |
Mycobacterium marinum | methyl 3-hydroxyoctanoate + S-adenosyl-L-homocysteine | - |
? | |
| decanoic acid + S-adenosyl-L-methionine | - |
Mycobacterium marinum | methyl decanoate + S-adenosyl-L-homocysteine | - |
? | |
| dodecanoic acid + S-adenosyl-L-methionine | - |
Mycobacterium marinum | methyl dodecanoate + S-adenosyl-L-homocysteine | - |
? | |
| octanoic acid + S-adenosyl-L-methionine | - |
Mycobacterium marinum | methyl octanoate + S-adenosyl-L-homocysteine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| fatty acid methyltransferase | - |
Mycobacterium marinum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 358 | - |
3-hydroxyoctanoic acid | pH 7.8, 37°C | Mycobacterium marinum | |
| 489 | - |
Decanoic acid | pH 7.8, 37°C | Mycobacterium marinum | |
| 510 | - |
octanoic acid | pH 7.8, 37°C | Mycobacterium marinum | |
| 1165 | - |
dodecanoic acid | pH 7.8, 37°C | Mycobacterium marinum | |
| 1346 | - |
3-hydroxydodecanoic acid | pH 7.8, 37°C | Mycobacterium marinum | |
| 1600 | - |
3-hydroxydecanoic acid | pH 7.8, 37°C | Mycobacterium marinum | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1820 | - |
3-hydroxyoctanoic acid | pH 7.8, 37°C | Mycobacterium marinum | |
| 6600 | - |
octanoic acid | pH 7.8, 37°C | Mycobacterium marinum | |
| 10400 | - |
3-hydroxydodecanoic acid | pH 7.8, 37°C | Mycobacterium marinum | |
| 10600 | - |
Decanoic acid | pH 7.8, 37°C | Mycobacterium marinum | |
| 11300 | - |
dodecanoic acid | pH 7.8, 37°C | Mycobacterium marinum | |
| 16100 | - |
3-hydroxydecanoic acid | pH 7.8, 37°C | Mycobacterium marinum | |
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