Application | Comment | Organism |
---|---|---|
medicine | thymidylate synthase as a target for antitubercular drugs | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
histidine-tagged Mycobacterium tuberculosis ThyX is overexpressed from BL21-DE3 pLysS Escherichia coli | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-fluoro-2'-deoxyuridine 5'-monophosphate | FdUMP | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
dUMP | - |
Mycobacterium tuberculosis | |
0.004 | - |
5,10-methylenetetrahydrofolate | - |
Mycobacterium tuberculosis | |
0.047 | - |
NADPH | - |
Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
104000 | - |
gel filtration | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP + FADH2 | Mycobacterium tuberculosis | - |
dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + NADPH | Mycobacterium tuberculosis | Rhodobacter capsulatus ThyX is required for de novo thymidylate synthesis | dTMP + tetrahydrofolate + NADP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity and ion-exchange chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Mycobacterium tuberculosis | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + NADPH | - |
Mycobacterium tuberculosis | dTMP + tetrahydrofolate + NADP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + NADPH | Rhodobacter capsulatus ThyX is required for de novo thymidylate synthesis | Mycobacterium tuberculosis | dTMP + tetrahydrofolate + NADP | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | predicted mass of a tetramer | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
FDTS | - |
Mycobacterium tuberculosis |
flavin-dependent thymidylate synthase | - |
Mycobacterium tuberculosis |
ThyX | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Mycobacterium tuberculosis | |
NADPH | - |
Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0001 | - |
5-fluoro-2'-deoxyuridine 5'-monophosphate | inhibited both ThyA and ThyX | Mycobacterium tuberculosis |