Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
24(28)-methylene cycloartanol | competitive | Glycine max | |
25-azacycloartanol | non-competitive | Glycine max | |
25-azacyloartenol | - |
Glycine max | |
sitosterol | competitive | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
Cycloartenol | - |
Glycine max | |
0.028 | - |
24(28)methylene lophenol | - |
Glycine max | |
0.03 | - |
Cycloartenol | - |
Glycine max | |
0.03 | - |
24(28)-methylene lophenol | - |
Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
4 * 40340, calculated, 4 * 40000, SDS-PAGE | Glycine max |
40340 | - |
4 * 40340, calculated, 4 * 40000, SDS-PAGE | Glycine max |
40397 | - |
x * 40397, calculated | Glycine max |
160000 | - |
gel filtration | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
isoform SMT2-1 | - |
Glycine max | D2D5G4 | isoform SMT2-2 | - |
Glycine max | Q43445 | - |
- |
Purification (Comment) | Organism |
---|---|
isoform SMT2-2 is purified to homogeneity by column chromatography, gel filtration | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Glycine max | - |
leaf | young leaf | Glycine max | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
24(28)-methylene lophenol + S-adenosyl-L-methionine | - |
Glycine max | ? | - |
? | |
24(28)-methylene lophenol + S-adenosyl-L-methionine | - |
Glycine max | 24(28)Z-ethylidene lophenol + S-adenosyl-L-homocysteine | - |
? | |
cycloartenol + S-adenosyl-L-methionine | - |
Glycine max | 24-methylenecycloartanol + S-adenosyl-L-homocysteine | - |
? | |
cycloartenol + S-adenosyl-L-methionine | 64% of the effectiveness with 24(28)-methylene lophenol | Glycine max | 24-methylenecycloartanol + S-adenosyl-L-homocysteine | - |
? | |
cycloartenol + S-adenosyl-L-methionine | optimal substrate for SMT1 | Glycine max | 24(28)-methylene cycloartanol + S-adenosyl-L-homocysteine | - |
? | |
ergosta-7,24(28)-dienol + S-adenosyl-L-methionine | fecosterol and ergosta-7,24(28)-dienol which lack C4-and C14-methyl groups in the nucleus are catalyzed equally effectively by SMT2-2 as the natural substrate 24(28)-methylene lophenol | Glycine max | ? + S-adenosyl-L-homocysteine | - |
? | |
fecosterol + S-adenosyl-L-methionine | fecosterol and ergosta-7,24(28)-dienol which lack C4-and C14-methyl groups in the nucleus are catalyzed equally effectively by SMT2-2 as the natural substrate 24(28)-methylene lophenol | Glycine max | ? + S-adenosyl-L-homocysteine | - |
? | |
lanosterol + S-adenosyl-L-methionine | - |
Glycine max | ? + S-adenosyl-L-homocysteine | - |
? | |
zymosterol + S-adenosyl-L-methionine | - |
Glycine max | fecosterol + S-adenosyl-L-homocysteine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40397, calculated | Glycine max |
tetramer | 4 * 40340, calculated, 4 * 40000, SDS-PAGE | Glycine max |
Synonyms | Comment | Organism |
---|---|---|
SMT1 | - |
Glycine max |
sterol 24C-methyltransferases | - |
Glycine max |
zymosterol-24(25)-methyltransferase | - |
Glycine max |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
Cycloartenol | - |
Glycine max | |
0.013 | - |
24(28)methylene lophenol | - |
Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Glycine max |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Glycine max |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00001 | - |
25-azacycloartanol | substrate cycloartenol | Glycine max | |
0.00002 | - |
25-azacycloartanol | substrate 24(28)-methylene lophenol | Glycine max | |
0.00004 | - |
25-azacyloartenol | - |
Glycine max | |
0.00009 | - |
24(28)-methylene cycloartanol | - |
Glycine max | |
0.0001 | - |
sitosterol | - |
Glycine max | |
0.077 | - |
24(28)-methylene cycloartanol | substrate cycloartenol | Glycine max | |
0.1 | - |
sitosterol | substrate cycloartenol | Glycine max | |
0.116 | - |
24(28)-methylene cycloartanol | substrate 24(28)-methylene lophenol | Glycine max | |
0.154 | - |
sitosterol | substrate 24(28)-methylene lophenol | Glycine max |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Glycine max | calculated | - |
6.3 |
Glycine max | calculated | - |
6.5 |