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Literature summary for 2.1.1.140 extracted from

  • Loeffler, S.; Deus-Neumann, B.; Zenk, M.H.
    S-Adenosyl-L-methionine:(S)-coclaurine-N-methyltransferase from Tinospora cordifolia (1995), Phytochemistry, 38, 1387-1395.
No PubMed abstract available
Search for more literature for 2.1.1.140

Inhibitors

Inhibitors Comment Organism Structure
S-adenosyl-L-methionine 50% inhibition at 0.062 mM, substrate (S)-coclaurine Tinospora cordifolia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 S-adenosyl-L-methionine
-
 Tinospora cordifolia
0.04
-
 (S)-Coclaurine
-
 Tinospora cordifolia
0.05
-
 (S)-norcoclaurine probably allosteric enzyme, Hill coeffizient 2.6 Tinospora cordifolia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83000
-
 gel filtration HPLC Tinospora cordifolia
87000
-
 gel filtration Tinospora cordifolia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-coclaurine + S-adenosyl-L-methionine Eschscholzia californica
-
 S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine Lamprocapnos spectabilis
-
 S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine Fumaria officinalis
-
 S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine Berberis koetineana
-
 S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine Berberis stolonifera
-
 S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine Bocconia cordata
-
 S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine Corydalis vaginans
-
 S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine Stephania delavayi
-
 S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine Tinospora cordifolia
-
 S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine Berberis canadensis
-
 S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine Cocculus trilobus
-
 S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine Tinospora fragosa
-
 S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine Thalictrum dasycarpum
-
 S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine Thalictrum flavum
-
 S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine Thalictrum glaucum
-
 S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?

Organism

Organism UniProt Comment Textmining
Berberis canadensis
-
-
-
Berberis koetineana
-
-
-
Berberis stolonifera
-
-
-
Bocconia cordata
-
-
-
Cocculus trilobus
-
-
-
Corydalis vaginans
-
-
-
Eschscholzia californica
-
-
-
Fumaria officinalis
-
-
-
Lamprocapnos spectabilis
-
-
-
Stephania delavayi
-
-
-
Thalictrum dasycarpum
-
-
-
Thalictrum flavum
-
-
-
Thalictrum glaucum
-
-
-
Tinospora cordifolia
-
-
-
Tinospora fragosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
40% ammonium sulfate, Phenyl Sepharose, TSK Fraktogel, Mono Q, Affinity gel, disc electrophoresis, 115fold purification Tinospora cordifolia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000096
-
 enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated Lamprocapnos spectabilis
0.000096
-
 enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated Fumaria officinalis
0.0001
-
 enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated Stephania delavayi
0.0003
-
 enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated Thalictrum flavum
0.00065
-
 enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated Thalictrum glaucum
0.00066
-
 enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated Thalictrum dasycarpum
0.0007
-
 enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated Corydalis vaginans
0.00075
-
 enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated Bocconia cordata
0.00077
-
 enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated Cocculus trilobus
0.0008
-
 enzyme extract of cell culture, (S)-coclaurine Berberis stolonifera
0.0013
-
 enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated Eschscholzia californica
0.002
-
 enzyme extract of cell culture, (S)-coclaurine Berberis koetineana
0.002
-
 enzyme extract of cell culture, (S)-coclaurine Berberis canadensis
0.003
-
 enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated Tinospora fragosa
0.0036
-
 enzyme extract of cell culture, (S)-coclaurine Tinospora cordifolia
0.4
-
-
 Tinospora cordifolia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-coclaurine + S-adenosyl-L-methionine
-
 Eschscholzia californica S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine
-
 Lamprocapnos spectabilis S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine
-
 Fumaria officinalis S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine
-
 Berberis koetineana S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine
-
 Berberis stolonifera S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine
-
 Bocconia cordata S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine
-
 Corydalis vaginans S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine
-
 Stephania delavayi S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine
-
 Tinospora cordifolia S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine
-
 Berberis canadensis S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine
-
 Cocculus trilobus S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine
-
 Tinospora fragosa S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine
-
 Thalictrum dasycarpum S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine
-
 Thalictrum flavum S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine
-
 Thalictrum glaucum S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-coclaurine + S-adenosyl-L-methionine strictly stereospecific, only (S)-coclaurine is N-methylated Tinospora cordifolia S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
(S)-norcoclaurine + S-adenosyl-L-methionine strictly stereospecific, only (S)-norcoclaurine is N-methylated Tinospora cordifolia S-adenosyl-L-homocysteine + (R)-N-methylnorcoclaurine
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
34 37 23fold purified enzyme preparation, pH 9.0 Tinospora cordifolia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
 narrow optimum Tinospora cordifolia

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5
-
 Tinospora cordifolia

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.062
-
 S-adenosyl-L-methionine substrate (S)-coclaurine Tinospora cordifolia