Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
V39A/R46C/T106I/K713E | mutant confers accelerated growth in the Escherichia coli K-12 WE strain in the presence of acetate. Strains harboring acetate-tolerant MetE mutants are less inhibited by homocysteine in L-isoleucine-enriched medium. The acetate-tolerant MetE mutants stimulate the growth of the host strain at elevated temperatures of 44 and 45°C. The mutant MetE enzymes display a reduced melting temperature but an enhanced in vivo stability | Escherichia coli |
V39A/R46C/T106I/K713E/C645A | C645A mutation additionally improves acetate tolerance. Strains harboring acetate-tolerant MetE mutants are less inhibited by homocysteine in L-isoleucine-enriched medium. The acetate-tolerant MetE mutants stimulate the growth of the host strain at elevated temperatures of 44 and 45°C. The mutant MetE enzymes display a reduced melting temperature but an enhanced in vivo stability | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P25665 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
MetE | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
48.5 | - |
melting temperature, mutant V39A/R46C/T106I/K713E/C645A | Escherichia coli |
51.3 | - |
melting temperature, mutant V39A/R46C/T106I/K713E | Escherichia coli |
54.7 | - |
melting temperature, wild-type | Escherichia coli |