Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.137 extracted from

  • Ding, L.; Saunders, R.J.; Drobna, Z.; Walton, F.S.; Xun, P.; Thomas, D.J.; Styblo, M.
    Methylation of arsenic by recombinant human wild-type arsenic (+3 oxidation state) methyltransferase and its methionine 287 threonine (M287T) polymorph: Role of glutathione (2012), Toxicol. Appl. Pharmacol., 264, 121-130.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
glutathione 1 mM GSH decreases Km and increases Vmax estimates for arsenite and methylarsonous acid Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
M287T in the absence of glutathione, the mutant shows decreased Vmax and Km for arsenite and increased Vmax and Km for methylarsonate compared to the wild type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
glutathione addition of 20 mM GSH sharply inhibits methylation Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0007
-
methylarsonate wild type enzyme, in the presence of 1 mM glutathione, in 100 mM Tris-HCl buffer (pH 7.4), at 25°C Homo sapiens
0.0009
-
methylarsonate mutant enzyme M287T, in the presence of 1 mM glutathione, in 100 mM Tris-HCl buffer (pH 7.4), at 25°C Homo sapiens
0.0015
-
methylarsonate wild type enzyme, in 100 mM Tris-HCl buffer (pH 7.4), at 25°C Homo sapiens
0.0016
-
arsenite mutant enzyme M287T, in the presence of 1 mM glutathione, in 100 mM Tris-HCl buffer (pH 7.4), at 37°C Homo sapiens
0.0016
-
arsenite wild type enzyme, in the presence of 1 mM glutathione, in 100 mM Tris-HCl buffer (pH 7.4), at 37°C Homo sapiens
0.002
-
arsenite mutant enzyme M287T, in 100 mM Tris-HCl buffer (pH 7.4), at 37°C Homo sapiens
0.003
-
methylarsonate mutant enzyme M287T, in 100 mM Tris-HCl buffer (pH 7.4), at 25°C Homo sapiens
0.0041
-
arsenite wild type enzyme, in 100 mM Tris-HCl buffer (pH 7.4), at 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + arsenite
-
Homo sapiens S-adenosyl-L-homocysteine + methylarsonate + dimethylarsinous acid + dimethylarsinic acid
-
?
S-adenosyl-L-methionine + methylarsonate
-
Homo sapiens S-adenosyl-L-homocysteine + dimethylarsinous acid + dimethylarsinic acid
-
?

Synonyms

Synonyms Comment Organism
arsenic (+3 oxidation state) methyltransferase
-
Homo sapiens
AS3MT
-
Homo sapiens

General Information

General Information Comment Organism
metabolism key enzyme in the pathway for methylation of arsenicals Homo sapiens