Literature summary for 2.1.1.136 extracted from

Feltrer, R.; Alvarez-Rodriguez, M.L.; Barreiro, C.; Godio, R.P.; Coque, J.J.
Characterization of a novel 2,4,6-trichlorophenol-inducible gene encoding chlorophenol O-methyltransferase from Trichoderma longibrachiatum responsible for the formation of chloroanisoles and detoxification of chlorophenols (2010), Fungal Genet. Biol., 47, 458-467.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Trichoderma longibrachiatum

Protein Variants

Protein Variants	Comment	Organism
additional information	partial silencing of cmt1 gene results in a 48.9% decrease of enzyme activity levels in a Trichoderma longibrachiatum At37 transformant strain by comparison with the wild type	Trichoderma longibrachiatum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
52400	-	x * 52400, deduced from amino acid sequence	Trichoderma longibrachiatum
65400	-	x * 65400, fusion protein SUMO-CMT1, SDS-PAGE	Trichoderma longibrachiatum

Organism

Organism	UniProt	Comment	Textmining
Trichoderma longibrachiatum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE column chromatography. hydroxyapatite column chromatography, Resource Q column chromatography, ultrafiltration, and Superdex 200 gel filtration	Trichoderma longibrachiatum

Source Tissue

Source Tissue	Comment	Organism	Textmining
mycelium	-	Trichoderma longibrachiatum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + 2,4,6-trichlorophenol	-	Trichoderma longibrachiatum	S-adenosyl-L-homocysteine + 2,4,6-trichloroanisole	-	?

Subunits

Subunits	Comment	Organism
?	x * 52400, deduced from amino acid sequence	Trichoderma longibrachiatum
?	x * 65400, fusion protein SUMO-CMT1, SDS-PAGE	Trichoderma longibrachiatum

Synonyms

Synonyms	Comment	Organism
CMT1	formerly named CPOMT	Trichoderma longibrachiatum

pI Value

Organism	Comment	pI Value Maximum	pI Value
Trichoderma longibrachiatum	deduced from amino acid sequence	-	5.9

Expression

Organism	Comment	Expression
Trichoderma longibrachiatum	the expression of the enzyme is induced at transcriptional level by exposure of fungal mycelia to 0.01 mg/ml 2,4,6-trichlorophenol	up

General Information

General Information	Comment	Organism
physiological function	the enzyme is responsible for the detoxification of chlorophenolic pesticides by O-methylation which results in the formation of chloroanisoles	Trichoderma longibrachiatum

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Release 2023.1 (January 2023)