General Stability | Organism |
---|---|
acetone inactivates | Sus scrofa |
freezing and thawing, 40% loss of activity | Sus scrofa |
mercaptoethanol, 0.2 M, inactivation after 2 h at 24°C or during freezing and thawing, no effect at 0°C | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-methyltetrahydrofolate + L-homocysteine | Sus scrofa | methionine biosynthesis | tetrahydrofolate + L-methionine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
assay method | Sus scrofa |
Storage Stability | Organism |
---|---|
0°C, glutathione, N2-atmosphere, stable for at least a week | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-methyltetrahydrofolate + L-homocysteine | methionine biosynthesis | Sus scrofa | tetrahydrofolate + L-methionine | - |
? | |
N5-methyltetrahydropteroylmonoglutamate + L-homocysteine | - |
Sus scrofa | tetrahydropteroylmonoglutamate + L-methionine | - |
? | |
N5-methyltetrahydropteroyltriglutamate + L-homocysteine | - |
Sus scrofa | tetrahydropteroyltriglutamate + L-methionine | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
2 h, complete loss of activity | Sus scrofa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
unstable | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | required | Sus scrofa | |
vitamin B12 | enzyme contains a derivative of vitamin B12 as prosthetic group | Sus scrofa |