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Literature summary for 2.1.1.13 extracted from

  • Buchanan, J.M.
    Methionine biosynthesis (hog liver) (1971), Methods Enzymol., 17B, 371-378.
No PubMed abstract available

General Stability

General Stability Organism
acetone inactivates Sus scrofa
freezing and thawing, 40% loss of activity Sus scrofa
mercaptoethanol, 0.2 M, inactivation after 2 h at 24°C or during freezing and thawing, no effect at 0°C Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-methyltetrahydrofolate + L-homocysteine Sus scrofa methionine biosynthesis tetrahydrofolate + L-methionine
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
assay method Sus scrofa

Storage Stability

Storage Stability Organism
0°C, glutathione, N2-atmosphere, stable for at least a week Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-methyltetrahydrofolate + L-homocysteine methionine biosynthesis Sus scrofa tetrahydrofolate + L-methionine
-
?
N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
Sus scrofa tetrahydropteroylmonoglutamate + L-methionine
-
?
N5-methyltetrahydropteroyltriglutamate + L-homocysteine
-
Sus scrofa tetrahydropteroyltriglutamate + L-methionine
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
2 h, complete loss of activity Sus scrofa

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
unstable Sus scrofa

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine required Sus scrofa
vitamin B12 enzyme contains a derivative of vitamin B12 as prosthetic group Sus scrofa