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Literature summary for 2.1.1.129 extracted from

  • Sengupta, S.; Patra, B.; Ray, S.; Majumder, A.L.
    Inositol methyl tranferase from a halophytic wild rice, Porteresia coarctata Roxb. (Tateoka): regulation of pinitol synthesis under abiotic stress (2008), Plant Cell Environ., 31, 1442-1459.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21DE3 PLys(S) strain, pET15b vector Oryza coarctata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
40 kDa immunoreactive protein under stressed and unstressed conditions in wild rice, an about 60 kDa major band detected by antibodies in domesticated and in wild rice Oryza coarctata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + myo-inositol Oryza coarctata
-
S-adenosyl-L-homocysteine + 1D-4-O-methyl-myo-inositol
-
?

Organism

Organism UniProt Comment Textmining
no activity in Oryza sativa
-
-
-
Oryza coarctata
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Roxb. Tateoka
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration and SDS-PAGE, recombinant protein Oryza coarctata

Source Tissue

Source Tissue Comment Organism Textmining
plantlet abiotic stress treatment Oryza coarctata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
proteome profile during salt-stress, up-regulation of RNA and protein expression following exposure to salinity, profiling of reaction product by gas chromatography, functional characterization of recombinant protein, regulation of pinitol synthesis pathway under different abiotic stresses, different plant species known to produce pinitol in response to stress listed, accumulation of D-pinitol via inositol is a stress-regulated pathway, presence of D-pinitol synthesizing protein/gene in a wild halophytic rice, absence in domesticated rice, adaptive feature in salt tolerance Oryza coarctata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + myo-inositol
-
Oryza coarctata S-adenosyl-L-homocysteine + 1D-4-O-methyl-myo-inositol
-
?
S-adenosyl-L-methionine + myo-inositol accumulation of methylated inositol in plants under salt-stress Oryza coarctata S-adenosyl-L-homocysteine + 1D-4-O-methyl-myo-inositol
-
?

Synonyms

Synonyms Comment Organism
PcIMT1
-
Oryza coarctata