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Literature summary for 2.1.1.127 extracted from

  • Mininno, M.; Brugiere, S.; Pautre, V.; Gilgen, A.; Ma, S.; Ferro, M.; Tardif, M.; Alban, C.; Ravanel, S.
    Characterization of chloroplastic fructose 1,6-bisphosphate aldolases as lysine-methylated proteins in plants (2012), J. Biol. Chem., 287, 21034-21044.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta-2 cells Pisum sativum
expressed in Escherichia coli Rosetta-2 cells Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
S-adenosyl-L-homocysteine
-
Arabidopsis thaliana
S-adenosyl-L-homocysteine
-
Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0045
-
[large subunit of Rubisco]-L-lysine at pH 7.8 and 30°C Pisum sativum
0.0129
-
[chloroplastic fructose 1,6-bisphosphate aldolase isoform 2]-L-lysine at pH 7.8 and 30°C Pisum sativum
0.0138
-
[chloroplastic fructose 1,6-bisphosphate aldolase isoform 2]-L-lysine at pH 7.8 and 30°C Arabidopsis thaliana
0.014
-
[chloroplastic fructose 1,6-bisphosphate aldolase isoform 3]-L-lysine at pH 7.8 and 30°C Pisum sativum
0.0148
-
[chloroplastic fructose 1,6-bisphosphate aldolase isoform 3]-L-lysine at pH 7.8 and 30°C Arabidopsis thaliana
0.314
-
[gamma-tocopherol methyltransferase]-L-lysine at pH 7.8 and 30°C Arabidopsis thaliana
0.32
-
[gamma-tocopherol methyltransferase]-L-lysine at pH 7.8 and 30°C Pisum sativum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54600
-
x * 54600, calculated from amino acid sequence Pisum sativum
54600
-
x * 54600, calculated from amino acid sequence Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arabidopsis thaliana in Arabidopsis, the large subunit of Rubisco is not naturally methylated. LSMT-L is able to interact with unmethylated Rubisco, but the complex is catalytically unproductive ?
-
?
S-adenosyl-L-homocysteine + [gamma-tocopherol methyltransferase]-L-lysine Pisum sativum
-
S-adenosyl-L-homocysteine [gamma-tocopherol methyltransferase]-N6-methyl-L-lysine
-
?
S-adenosyl-L-methionine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 2]-L-lysine Pisum sativum
-
S-adenosyl-L-homocysteine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 2]-N6-methyl-L-lysine
-
?
S-adenosyl-L-methionine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 2]-L-lysine Arabidopsis thaliana
-
S-adenosyl-L-homocysteine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 2]-N6-methyl-L-lysine
-
?
S-adenosyl-L-methionine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 3]-L-lysine Pisum sativum
-
S-adenosyl-L-homocysteine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 3]-N6-methyl-L-lysine
-
?
S-adenosyl-L-methionine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 3]-L-lysine Arabidopsis thaliana
-
S-adenosyl-L-homocysteine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 3]-N6-methyl-L-lysine
-
?
S-adenosyl-L-methionine + [gamma-tocopherol methyltransferase]-L-lysine Arabidopsis thaliana
-
S-adenosyl-L-homocysteine + [gamma-tocopherol methyltransferase]-N6-methyl-L-lysine
-
?
S-adenosyl-L-methionine + [large subunit of Rubisco]-L-lysine Pisum sativum methylation occurs at Lys-394 S-adenosyl-L-homocysteine + [large subunit of Rubisco]-N6-methyl-L-lysine
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
ecotype Col-0
-
Pisum sativum
-
cultivar Douce Provence
-

Purification (Commentary)

Purification (Comment) Organism
nickel-Sepharose column chromatography, Sephadex G-25 gel filtration, and Q-Sepharose column chromatography Pisum sativum
nickel-Sepharose column chromatography, Sephadex G-25 gel filtration, and Q-Sepharose column chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Pisum sativum
-
leaf
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information in Arabidopsis, the large subunit of Rubisco is not naturally methylated. LSMT-L is able to interact with unmethylated Rubisco, but the complex is catalytically unproductive Arabidopsis thaliana ?
-
?
S-adenosyl-L-homocysteine + [gamma-tocopherol methyltransferase]-L-lysine
-
Pisum sativum S-adenosyl-L-homocysteine [gamma-tocopherol methyltransferase]-N6-methyl-L-lysine
-
?
S-adenosyl-L-methionine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 2]-L-lysine
-
Pisum sativum S-adenosyl-L-homocysteine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 2]-N6-methyl-L-lysine
-
?
S-adenosyl-L-methionine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 2]-L-lysine
-
Arabidopsis thaliana S-adenosyl-L-homocysteine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 2]-N6-methyl-L-lysine
-
?
S-adenosyl-L-methionine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 3]-L-lysine
-
Pisum sativum S-adenosyl-L-homocysteine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 3]-N6-methyl-L-lysine
-
?
S-adenosyl-L-methionine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 3]-L-lysine
-
Arabidopsis thaliana S-adenosyl-L-homocysteine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 3]-N6-methyl-L-lysine
-
?
S-adenosyl-L-methionine + [gamma-tocopherol methyltransferase]-L-lysine
-
Arabidopsis thaliana S-adenosyl-L-homocysteine + [gamma-tocopherol methyltransferase]-N6-methyl-L-lysine
-
?
S-adenosyl-L-methionine + [large subunit of Rubisco]-L-lysine methylation occurs at Lys-394 Pisum sativum S-adenosyl-L-homocysteine + [large subunit of Rubisco]-N6-methyl-L-lysine
-
?

Subunits

Subunits Comment Organism
? x * 54600, calculated from amino acid sequence Pisum sativum
? x * 54600, calculated from amino acid sequence Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
LSMT
-
Pisum sativum
LSMT-L
-
Arabidopsis thaliana
protein-lysine methyltransferase
-
Pisum sativum
protein-lysine methyltransferase-like
-
Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.033
-
[gamma-tocopherol methyltransferase]-L-lysine at pH 7.8 and 30°C Arabidopsis thaliana
0.05
-
[gamma-tocopherol methyltransferase]-L-lysine at pH 7.8 and 30°C Pisum sativum
0.57
-
[chloroplastic fructose 1,6-bisphosphate aldolase isoform 3]-L-lysine at pH 7.8 and 30°C Pisum sativum
0.933
-
[chloroplastic fructose 1,6-bisphosphate aldolase isoform 3]-L-lysine at pH 7.8 and 30°C Arabidopsis thaliana
1.05
-
[chloroplastic fructose 1,6-bisphosphate aldolase isoform 2]-L-lysine at pH 7.8 and 30°C Arabidopsis thaliana
1.05
-
[chloroplastic fructose 1,6-bisphosphate aldolase isoform 3]-L-lysine at pH 7.8 and 30°C Pisum sativum
1.32
-
[chloroplastic fructose 1,6-bisphosphate aldolase isoform 2]-L-lysine at pH 7.8 and 30°C Pisum sativum
8.65
-
[large subunit of Rubisco]-L-lysine at pH 7.8 and 30°C Pisum sativum