Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta-2 cells | Pisum sativum |
expressed in Escherichia coli Rosetta-2 cells | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-homocysteine | - |
Arabidopsis thaliana | |
S-adenosyl-L-homocysteine | - |
Pisum sativum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
[large subunit of Rubisco]-L-lysine | at pH 7.8 and 30°C | Pisum sativum | |
0.0129 | - |
[chloroplastic fructose 1,6-bisphosphate aldolase isoform 2]-L-lysine | at pH 7.8 and 30°C | Pisum sativum | |
0.0138 | - |
[chloroplastic fructose 1,6-bisphosphate aldolase isoform 2]-L-lysine | at pH 7.8 and 30°C | Arabidopsis thaliana | |
0.014 | - |
[chloroplastic fructose 1,6-bisphosphate aldolase isoform 3]-L-lysine | at pH 7.8 and 30°C | Pisum sativum | |
0.0148 | - |
[chloroplastic fructose 1,6-bisphosphate aldolase isoform 3]-L-lysine | at pH 7.8 and 30°C | Arabidopsis thaliana | |
0.314 | - |
[gamma-tocopherol methyltransferase]-L-lysine | at pH 7.8 and 30°C | Arabidopsis thaliana | |
0.32 | - |
[gamma-tocopherol methyltransferase]-L-lysine | at pH 7.8 and 30°C | Pisum sativum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54600 | - |
x * 54600, calculated from amino acid sequence | Pisum sativum |
54600 | - |
x * 54600, calculated from amino acid sequence | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | in Arabidopsis, the large subunit of Rubisco is not naturally methylated. LSMT-L is able to interact with unmethylated Rubisco, but the complex is catalytically unproductive | ? | - |
? | |
S-adenosyl-L-homocysteine + [gamma-tocopherol methyltransferase]-L-lysine | Pisum sativum | - |
S-adenosyl-L-homocysteine [gamma-tocopherol methyltransferase]-N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 2]-L-lysine | Pisum sativum | - |
S-adenosyl-L-homocysteine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 2]-N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 2]-L-lysine | Arabidopsis thaliana | - |
S-adenosyl-L-homocysteine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 2]-N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 3]-L-lysine | Pisum sativum | - |
S-adenosyl-L-homocysteine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 3]-N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 3]-L-lysine | Arabidopsis thaliana | - |
S-adenosyl-L-homocysteine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 3]-N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + [gamma-tocopherol methyltransferase]-L-lysine | Arabidopsis thaliana | - |
S-adenosyl-L-homocysteine + [gamma-tocopherol methyltransferase]-N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + [large subunit of Rubisco]-L-lysine | Pisum sativum | methylation occurs at Lys-394 | S-adenosyl-L-homocysteine + [large subunit of Rubisco]-N6-methyl-L-lysine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
ecotype Col-0 | - |
Pisum sativum | - |
cultivar Douce Provence | - |
Purification (Comment) | Organism |
---|---|
nickel-Sepharose column chromatography, Sephadex G-25 gel filtration, and Q-Sepharose column chromatography | Pisum sativum |
nickel-Sepharose column chromatography, Sephadex G-25 gel filtration, and Q-Sepharose column chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Pisum sativum | - |
leaf | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | in Arabidopsis, the large subunit of Rubisco is not naturally methylated. LSMT-L is able to interact with unmethylated Rubisco, but the complex is catalytically unproductive | Arabidopsis thaliana | ? | - |
? | |
S-adenosyl-L-homocysteine + [gamma-tocopherol methyltransferase]-L-lysine | - |
Pisum sativum | S-adenosyl-L-homocysteine [gamma-tocopherol methyltransferase]-N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 2]-L-lysine | - |
Pisum sativum | S-adenosyl-L-homocysteine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 2]-N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 2]-L-lysine | - |
Arabidopsis thaliana | S-adenosyl-L-homocysteine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 2]-N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 3]-L-lysine | - |
Pisum sativum | S-adenosyl-L-homocysteine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 3]-N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 3]-L-lysine | - |
Arabidopsis thaliana | S-adenosyl-L-homocysteine + [chloroplastic fructose 1,6-bisphosphate aldolase isoform 3]-N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + [gamma-tocopherol methyltransferase]-L-lysine | - |
Arabidopsis thaliana | S-adenosyl-L-homocysteine + [gamma-tocopherol methyltransferase]-N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + [large subunit of Rubisco]-L-lysine | methylation occurs at Lys-394 | Pisum sativum | S-adenosyl-L-homocysteine + [large subunit of Rubisco]-N6-methyl-L-lysine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54600, calculated from amino acid sequence | Pisum sativum |
? | x * 54600, calculated from amino acid sequence | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
LSMT | - |
Pisum sativum |
LSMT-L | - |
Arabidopsis thaliana |
protein-lysine methyltransferase | - |
Pisum sativum |
protein-lysine methyltransferase-like | - |
Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
[gamma-tocopherol methyltransferase]-L-lysine | at pH 7.8 and 30°C | Arabidopsis thaliana | |
0.05 | - |
[gamma-tocopherol methyltransferase]-L-lysine | at pH 7.8 and 30°C | Pisum sativum | |
0.57 | - |
[chloroplastic fructose 1,6-bisphosphate aldolase isoform 3]-L-lysine | at pH 7.8 and 30°C | Pisum sativum | |
0.933 | - |
[chloroplastic fructose 1,6-bisphosphate aldolase isoform 3]-L-lysine | at pH 7.8 and 30°C | Arabidopsis thaliana | |
1.05 | - |
[chloroplastic fructose 1,6-bisphosphate aldolase isoform 2]-L-lysine | at pH 7.8 and 30°C | Arabidopsis thaliana | |
1.05 | - |
[chloroplastic fructose 1,6-bisphosphate aldolase isoform 3]-L-lysine | at pH 7.8 and 30°C | Pisum sativum | |
1.32 | - |
[chloroplastic fructose 1,6-bisphosphate aldolase isoform 2]-L-lysine | at pH 7.8 and 30°C | Pisum sativum | |
8.65 | - |
[large subunit of Rubisco]-L-lysine | at pH 7.8 and 30°C | Pisum sativum |