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Literature summary for 2.1.1.127 extracted from

  • Wang, P.; Royer, M.; Houtz, R.L.
    Affinity purification of ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit epsilonN-methyltransferase (1995), Protein Expr. Purif., 6, 528-536.
    View publication on PubMed
Search for more literature for 2.1.1.127

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
 ribulose-1,5-bisphosphate-carboxylase native enzyme Pisum sativum
0.00827
-
 S-adenosyl-L-methionine recombinant enzyme Pisum sativum
0.0083
-
 ribulose-1,5-bisphosphate-carboxylase recombinant enzyme Pisum sativum
0.0137
-
 S-adenosyl-L-methionine native enzyme Pisum sativum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 the full-length enzyme is processed by removing the N-terminal 36 amino acids when expressed in E. coli cells Pisum sativum
60000
-
 x * 60000, SDS-PAGE Pisum sativum

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
large scale affinity purification Pisum sativum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.3
-
-
 Pisum sativum

Storage Stability

Storage Stability Organism
-80°C, in presence of 2 mg/ml beta-lactoglobulin, indefinitely stable Pisum sativum
4°C, stable for up to 30 days Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + [ribulose-1,5-bisphosphate-carboxylase]-L-lysine catalyzes methylation of the epsilon-amino group of Lys-14 in the large subunit of ribulose-bisphosphate-carboxylase from Spinacia oleracea Pisum sativum S-adenosyl-L-homocysteine + [ribulose-1,5-bisphosphate-carboxylase]-N6-methyl-L-lysine
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Pisum sativum