Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | induced by elicitor prepared from mycelia of Botrytis cinerea | Papaver somniferum | |
additional information | induced by elicitor prepared from mycelia of Botrytis cinerea | Papaver bracteatum |
Application | Comment | Organism |
---|---|---|
additional information | low TNMT activity | Thalictrum flavum |
Cloned (Comment) | Organism |
---|---|
Escherichia coli strain ER2566 pLysS transformed with pTNMT plasmid | Papaver somniferum |
expressed in Escherichia coli ER2566 pLysS cells, pRSETB vector | Papaver somniferum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | inhibits activity by 62%, inhibition prevented by inclusion of 10 mM EDTA; inhibits TNMT activity by 62%, can be prevented by the inclusion of EDTA | Papaver somniferum | |
Co2+ | inhibits activity by 71%, inhibition prevented by inclusion of 10 mM EDTA; inhibits TNMT activity by 71%, can be prevented by the inclusion of EDTA | Papaver somniferum | |
Mn2+ | inhibits activity by 41%, inhibition prevented by inclusion of 10 mM EDTA; inhibits TNMT activity by 41%, can be prevented by the inclusion of EDTA | Papaver somniferum | |
additional information | not inhibited by MgCl2 | Papaver somniferum | |
Ni2+ | inhibits activity by 72%, inhibition prevented by inclusion of 10 mM EDTA; inhibits TNMT activity by 72%, can be prevented by the inclusion of EDTA | Papaver somniferum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
(S)-Stylopine | - |
Papaver somniferum | |
0.0006 | - |
(R,S)-stylopine | varying concentration from 0.5 to 32 microM at a saturating concentration of 20 microM of S-adenosyl-L-methionine | Papaver somniferum | |
0.0115 | - |
S-adenosyl-L-methionine | - |
Papaver somniferum | |
0.0115 | - |
S-adenosyl-L-methionine | varying concentration from 0.625 to 40 microM, at a saturating concentration of 50 microM of (R,S)-stylopine | Papaver somniferum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Papaver somniferum | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | no effect on activity | Papaver somniferum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
- |
Papaver somniferum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Argemone mexicana | - |
- |
- |
Chelidonium majus | - |
- |
- |
Eschscholzia californica | - |
- |
- |
no activity in Nandina domestica | - |
- |
- |
no activity in Nicotiana tabacum | - |
- |
- |
no activity in Tinospora cordifolia | - |
- |
- |
Papaver bracteatum | - |
- |
- |
Papaver somniferum | - |
- |
- |
Papaver somniferum | Q108P1 | cv. Marianne, opium poppy | - |
Sanguinaria canadensis | - |
- |
- |
Thalictrum flavum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by nickel affinity chromatography | Papaver somniferum |
recombinant protein, gel filtration and SDS-PAGE | Papaver somniferum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | elicitor treatment | Papaver somniferum | - |
flower bud | TNMTgene transcripts at lower levels, but lower levels of TNMT activity | Papaver somniferum | - |
leaf | TNMTgene transcripts to a lesser extent, but highest levels of TNMT activity | Papaver somniferum | - |
root | TNMTgene transcripts most abundantly, but lower levels of TNMT activity | Papaver somniferum | - |
seedling | neither TNMT transcripts nor enzyme activity in seedlings 1 day after seed inbibition, whereas both TNMT transcript levels and enzyme activity 4 days after inbibition, remaining relatively constant during seedling growth | Papaver somniferum | - |
seedling | transcripts and enzyme activity in | Papaver somniferum | - |
stem | TNMTgene transcripts at lower levels, but highest levels of TNMT activity | Papaver somniferum | - |
whole plant | transcripts and enzyme activity in | Papaver somniferum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
phylogenetic analysis to examine possible evolutionary relationships, strict substrate specificity of recombinant enzyme, expression induced in cultured cells after treatment with a fungal elicitor | Papaver somniferum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | dimethoxytetrahydroisoquinoline and methylisoquinolinediol, norlaudanosoline, (R,S)-tetrahydroxyberbine and (S)-scoulerine, or (R,S)-pavine are not accepted as substrates | Papaver somniferum | ? | - |
? | |
S-adenosyl-L-methionine + (R,S)-canadine | - |
Papaver somniferum | ? | - |
? | |
S-adenosyl-L-methionine + (R,S)-stylopine | - |
Sanguinaria canadensis | ? | - |
? | |
S-adenosyl-L-methionine + (R,S)-stylopine | - |
Eschscholzia californica | ? | - |
? | |
S-adenosyl-L-methionine + (R,S)-stylopine | - |
Papaver somniferum | ? | - |
? | |
S-adenosyl-L-methionine + (R,S)-stylopine | - |
Chelidonium majus | ? | - |
? | |
S-adenosyl-L-methionine + (R,S)-stylopine | - |
Papaver bracteatum | ? | - |
? | |
S-adenosyl-L-methionine + (R,S)-stylopine | - |
Argemone mexicana | ? | - |
? | |
S-adenosyl-L-methionine + (R,S)-stylopine | strict substrate specificity, protoberberine alkaloids tetrahydroxyberbine and scoulerine, simple isoquinoline, benzylisoquinoline, and pavine alkaloids not accepted as substrates | Papaver somniferum | ? | - |
? | |
S-adenosyl-L-methionine + (R,S)-tetrahydropalmatine | - |
Papaver somniferum | ? | - |
? | |
S-adenosyl-L-methionine + (R,S)-tetrahydropalmatine | used for product identification | Papaver somniferum | ? | - |
? | |
S-Adenosyl-L-methionine + (S)-stylopine | - |
Papaver somniferum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(S)-tetrahydroprotoberberine cis-N-methyltransferase | - |
Sanguinaria canadensis |
(S)-tetrahydroprotoberberine cis-N-methyltransferase | - |
Eschscholzia californica |
(S)-tetrahydroprotoberberine cis-N-methyltransferase | - |
Papaver somniferum |
(S)-tetrahydroprotoberberine cis-N-methyltransferase | - |
Chelidonium majus |
(S)-tetrahydroprotoberberine cis-N-methyltransferase | - |
Papaver bracteatum |
(S)-tetrahydroprotoberberine cis-N-methyltransferase | - |
Thalictrum flavum |
(S)-tetrahydroprotoberberine cis-N-methyltransferase | - |
Argemone mexicana |
S-adenosyl-L-methionine:tetrahydroprotoberberine cis-N-methyltransferase | - |
Sanguinaria canadensis |
S-adenosyl-L-methionine:tetrahydroprotoberberine cis-N-methyltransferase | - |
Eschscholzia californica |
S-adenosyl-L-methionine:tetrahydroprotoberberine cis-N-methyltransferase | - |
Papaver somniferum |
S-adenosyl-L-methionine:tetrahydroprotoberberine cis-N-methyltransferase | - |
Chelidonium majus |
S-adenosyl-L-methionine:tetrahydroprotoberberine cis-N-methyltransferase | - |
Papaver bracteatum |
S-adenosyl-L-methionine:tetrahydroprotoberberine cis-N-methyltransferase | - |
Thalictrum flavum |
S-adenosyl-L-methionine:tetrahydroprotoberberine cis-N-methyltransferase | - |
Argemone mexicana |
TNMT | - |
Sanguinaria canadensis |
TNMT | - |
Eschscholzia californica |
TNMT | - |
Papaver somniferum |
TNMT | - |
Chelidonium majus |
TNMT | - |
Papaver bracteatum |
TNMT | - |
Thalictrum flavum |
TNMT | - |
Argemone mexicana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Papaver somniferum |
37 | - |
assay at | Papaver somniferum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 50 | recombinant protein, half-maximal activity estimated at 12°C and 47°C | Papaver somniferum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Papaver somniferum |
7.5 | - |
determined by using 100 mM sodium phosphate (pH 5-7) and 100 mM Tris-HCl (pH 7-9) containing 12 mM beta-mercaptoethanol | Papaver somniferum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | recombinant protein, half-maximal activity estimated at pH 6.8 and 8.2 | Papaver somniferum |