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Literature summary for 2.1.1.121 extracted from
- A highly specific O-methyltransferase for nororientaline synthesis isolated from Argemone platyceras cell cultures (1983), Planta Med., 49, 196-198.
General Stability
| General Stability | Organism |
|---|---|
| complete inactivation by freezing even in presence of 30% glycerol | Argemone platyceras |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Fe3+ | 5 mM, complete inhibition | Argemone platyceras |  |
| Hg2+ | 5 mM, complete inhibition | Argemone platyceras | |
| iodobenzoate | 10 mM, 79% inhibition | Argemone platyceras | |
| NEM | 5 mM, 82% inhibition | Argemone platyceras | |
| PCMB | 5 mM, 84% inhibition | Argemone platyceras | |
| S-adenosyl-L-homocysteine | - |
Argemone platyceras | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
S-adenosyl-L-methionine | - |
Argemone platyceras | |
| 0.4 | - |
6-O-methylnorlaudanosoline | - |
Argemone platyceras | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-Adenosyl-L-methionine + 6-O-methylnorlaudanosoline | Argemone platyceras | the product nororientaline is a precursor of the alkaloid papaverine | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Argemone platyceras | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Argemone platyceras |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Argemone platyceras | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Argemone platyceras |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 0.05% NaN3, stable for 4 weeks | Argemone platyceras |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-Adenosyl-L-methionine + 6-O-methylnorlaudanosoline | the product nororientaline is a precursor of the alkaloid papaverine | Argemone platyceras | ? | - |
? | |
| S-Adenosyl-L-methionine + 6-O-methylnorlaudanosoline | - |
Argemone platyceras | S-Adenosyl-L-homocysteine + nororientaline | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Argemone platyceras |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
half-life: 33 h | Argemone platyceras |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Argemone platyceras |
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Release 2023.1 (January 2023)
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