Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 5 mM, 97% inhibition | Berberis wilsoniae | |
S-adenosylhomocysteine | - |
Berberis wilsoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
S-adenosyl-L-methionine | - |
Berberis wilsoniae | |
1.6 | - |
(S)-scoulerine | - |
Berberis wilsoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
- |
Berberis wilsoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-Adenosyl-L-methionine + (S)-scoulerine | Berberis wilsoniae | central role in orotoberberine biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Berberis wilsoniae | - |
var. subcauliata | - |
Purification (Comment) | Organism |
---|---|
- |
Berberis wilsoniae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Berberis wilsoniae | - |
cell suspension culture | - |
Berberis wilsoniae | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Berberis wilsoniae |
Storage Stability | Organism |
---|---|
-20°C, 20% glycerol, 50% loss of activity after 7 days | Berberis wilsoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-Adenosyl-L-methionine + (R,S)-2,3,9,10-tetrahydroxytetrahydroprotoberberine | methylated at 50% of the rate of (S)-scoulerine | Berberis wilsoniae | ? | - |
? | |
S-Adenosyl-L-methionine + (S)-3,9-dihydroxy-2,10-dimethoxytetrahydroprotoberberine | methylated at 4% of the rate of (S)-scoulerine | Berberis wilsoniae | ? | - |
? | |
S-Adenosyl-L-methionine + (S)-scoulerine | - |
Berberis wilsoniae | S-Adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine | - |
? | |
S-Adenosyl-L-methionine + (S)-scoulerine | central role in orotoberberine biosynthesis | Berberis wilsoniae | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Berberis wilsoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.9 | - |
- |
Berberis wilsoniae |