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Literature summary for 2.1.1.116 extracted from

  • Ziegler, J.; Diaz-Chavez, M.L.; Kramell, R.; Ammer, C.; Kutchan, T.M.
    Comparative macroarray analysis of morphine containing Papaver somniferum and eight morphine free Papaver species identifies an O-methyltransferase involved in benzylisoquinoline biosynthesis (2005), Planta, 222, 458-471.
    View publication on PubMed
Search for more literature for 2.1.1.116

Application

Application Comment Organism
additional information the fragment A11A1 with increased expression in Papaver somniferum plants compared to other Papaver species shows the highest homology (75% identity on protein level) to the 4'-OMT from Coptis japonica Papaver somniferum

Cloned(Commentary)

Cloned (Comment) Organism
into vector pQE31 containing six histidines as an N-terminal tag and overexpressed in Escherichia coli SG13009 Papaver somniferum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.031
-
 (R,S)-6-O-methylnorlaudanosoline
-
 Papaver somniferum
0.037
-
 (R,S)-norlaudanosoline
-
 Papaver somniferum
0.039
-
 (R,S)-laudanosoline
-
 Papaver somniferum
0.081
-
 (R,S)-3'-hydroxy-N-methylcoclaurine
-
 Papaver somniferum
0.136
-
 S-adenosyl-L-methionine with (R,S)-norlaudanosoline as substrate Papaver somniferum
0.179
-
 S-adenosyl-L-methionine with (R,S)-6-O-methylnorlaudanosoline as substrate Papaver somniferum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39800
-
 2 * 42500, SDS-PAGE, 2 * 39800, sequence analysis Papaver somniferum
42500
-
 2 * 42500, SDS-PAGE, 2 * 39800, sequence analysis Papaver somniferum
75000
-
 gel filtration Papaver somniferum

Organism

Organism UniProt Comment Textmining
Papaver somniferum
-
 varieties Paso Munich, Noscapine and NOPA
-

Purification (Commentary)

Purification (Comment) Organism
on cobalt affinity column Papaver somniferum

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Papaver somniferum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information only tetrahydroxylated tetrahydrobenzylisoquinolines are accepted as substrates, methylation is tolerated only at the 6-hydroxy position, no methylation when catechol and guaiacol or the phenylpropanoids caffeic and ferulic acid are used as substrate Papaver somniferum ?
-
 ?
S-adenosyl-L-methionine + (R,S)-3'-hydroxy-N-methylcoclaurine
-
 Papaver somniferum ?
-
 ?
S-adenosyl-L-methionine + (R,S)-6-O-methylnorlaudanosoline
-
 Papaver somniferum ?
-
 ?
S-adenosyl-L-methionine + (R,S)-laudanosoline
-
 Papaver somniferum ?
-
 ?
S-adenosyl-L-methionine + (R,S)-norlaudanosoline
-
 Papaver somniferum ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 42500, SDS-PAGE, 2 * 39800, sequence analysis Papaver somniferum

Synonyms

Synonyms Comment Organism
4'-OMT
-
 Papaver somniferum
S-adenosyl-L-methionine:(R,S)-3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase
-
 Papaver somniferum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 with (R,S)-3'-hydroxy-N-methylcoclaurine as substrate Papaver somniferum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 with (R,S)-3'-hydroxy-N-methylcoclaurine as substrate Papaver somniferum